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Plasmid#105602Purposeexpressed 7+ sortase A variant in E.coliDepositorInsertS.aureus Sortase A, 7+ mutant
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-IPP1-His
Plasmid#124137PurposeBacterial expression plasmid for PURE system componentsDepositorInsertInorganic pyrophosphatase (IPP1 Budding Yeast)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
His6-SUMO-pET28a-EfaCas1
Plasmid#112793PurposeTo express enterococcus faecalis Cas1 protein in E. coliDepositorInsertEfaCas1
UseTagsHis6-SUMOExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc
Plasmid#117728PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc geneDepositorInsertNanoLuc
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm-TetR-P1T_DP-GH023
Plasmid#90324PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoterDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Rtn3L-mNeonGreen
Plasmid#169034PurposeExpress human Rtn3L in mammalian cellsDepositorInsertRtn3L (RTN3 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
iMbody_pSANG10-3F
Plasmid#184496PurposeExpresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteriaDepositorInsertiMbody
UseTags3xFLAG, 6xHis, and pelB leaderExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-mCherry-NS
Plasmid#108885PurposeMultisite gateway vector for 5' tagging with mCherryDepositorInsertmCherry
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSALECT-EcoBam
Plasmid#59705PurposeFolding reporter with ampicillin resistance as a readoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
H3K9me3Probe_FullLength
Plasmid#186463PurposeExpresses GFP tagged H3K9me3 recognition motif in mammalian cells.DepositorInsertdimeric CDY1 (CDY1 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:turquoise2
Plasmid#135218PurposeCyan tagged macrophagesDepositorInsertmfap4 promoter and mTurquoise2
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-eGFP-MCS-polyA
Plasmid#174024PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInserteGFP
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
APEX2-csChBP
Plasmid#108876PurposeExpression of APEX2 fused to a conditionally stable nanobody against mCherryDepositorInsertAPEX2-csChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp Phy-mCherry-CAAX
Plasmid#50839PurposeOptogenetic PI3K activation system; membrane componentDepositorInsertPhyB 1-908
UseLentiviral and Synthetic BiologyTagsmCherryExpressionMutationPromoterSFFVAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-) EC Her2-Fc
Plasmid#183250PurposeSoluble expression of the extracellular domain of the HER2 antigen. Human IgG1 Fc.DepositorInsertHer2 antigen Fc
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-FLAG-SENP3
Plasmid#115521PurposeMammalian expression constructDepositorInsertSENP3 (Senp3 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR iSH-YFP-PIF
Plasmid#50841PurposeOptogenetic PI3K activation system; cytoplasmic componentDepositorInsertsp85 iSH2
PIF6 1-100
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
ecAT3.10
Plasmid#107215PurposeMammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.DepositorInsertecAT3.10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
csr-1_hH1_mEos3.1_V5
Plasmid#191750PurposePhotoconvertible fluorescent nuclear reporter.DepositorInserthH1 (hH1 Neurospora crassa)
UseTagsV5, ccg-1 promoter, and mEos3.1ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only