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pSGE_764_4xUAS_DOS_hkCP
Plasmid#71172PurposeLuciferase reporterDepositorInsert764_4xUAS_DOS_hkCP
UseLuciferaseTagsluciferaseExpressionInsectMutationPromoterDSCP Drosophila synthetic core promoterAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Endosomes-14
Plasmid#55293PurposeLocalization: Endosomes, Excitation: 399, Emission: 456DepositorInsertEndosomes (RHOB Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagRFP-T-Endosomes-14
Plasmid#58014PurposeLocalization: Endosomes, Excitation: 555, Emission: 584DepositorInsertEndosomes (RHOB Human)
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
EGFP-Endosomes-13
Plasmid#56433PurposeLocalization: Endosomes, Excitation: 488, Emission: 507DepositorInsertEndosomes (RHOB Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EYFP-Endosomes-13
Plasmid#56588PurposeLocalization: Endosomes, Excitation: 513, Emission: 527DepositorInsertEndosomes (RHOB Human)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEXndoS(E235Q)
Plasmid#44656DepositorInsertndoS (ndoS Streptococcus pyogenes)
UseTagsGstExpressionBacterialMutationChanged Glutamic acid 235 to GlutaminePromotertacAvailable sinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
Endosome-targeted optoPLD
Plasmid#140056PurposeEncodes CRY2-mCherry-PLD-P2A-CIBN-2xFYVE for mammalian expressionDepositorInsertPhospholipase D
UseTags2xFYVE, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Endosome-targeted optodPLD
Plasmid#140055PurposeEncodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expressionDepositorInsertPhospholipase D
UseTags2xFYVE, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationH170A (catalytic dead)PromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Endosomal Escape Control
Plasmid#186552PurposeNegative Control confirming the need for a CPP peptide in mediating internalizationDepositorInsert6H-ETA-GFP
UseTags6xHis and EGFPExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization and Endosomal Escape
Plasmid#186551PurposeInternalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cellsDepositorInsert10R-8His-ETA-EGFP
UseTags8xHis and EGFPExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CRY2-mCherry
Plasmid#58368PurposeMamlian codon optimized CRY2DepositorInsertCRY2[1-498] (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-monSTIM1
Plasmid#161795PurposeMammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)DepositorInsertMonSTIM1 (STIM1 Human, Mustard Weed)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-OptoFGFR1
Plasmid#59776PurposeExpresses myristoylation signal peptide-FGFR1 cytoplasmic region-PHR domain of cryptochrome2-mCitrine fused proteinDepositorInsertOptoFGFR1 (FGFR1 Human, Mustard Weed)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-Beclin 1
Plasmid#86748PurposeN-terminal HA-tagged protein expression in mammalian cellsDepositorInserthuman Beclin 1 (BECN1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-G-Kras
Plasmid#180571PurposeddGFP sensor of KrasDepositorInsertddGFP sensor of Kras
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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myc-Atg14 (71-181)
Plasmid#86753PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 71-181PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (396-492)
Plasmid#86755PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 396-492PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (181-413)
Plasmid#86754PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 181-413PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only
