pCDNA3-FLAG-SENP3
(Plasmid
#115521)
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PurposeMammalian expression construct
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 115521 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSENP3
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SpeciesM. musculus (mouse)
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Insert Size (bp)1700
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Entrez GeneSenp3 (a.k.a. AA408656, Smt3ip, Smt3ip1)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3-FLAG-SENP3 was a gift from Martine Roussel (Addgene plasmid # 115521 ; http://n2t.net/addgene:115521 ; RRID:Addgene_115521) -
For your References section:
Arf-induced turnover of the nucleolar nucleophosmin-associated SUMO-2/3 protease Senp3. Kuo ML, den Besten W, Thomas MC, Sherr CJ. Cell Cycle. 2008 Nov 1;7(21):3378-87. doi: 10.4161/cc.7.21.6930. Epub 2008 Nov 4. 10.4161/cc.7.21.6930 PubMed 18948745
Map uploaded by the depositor.
