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pGLS3-5xHRE-p53(K120R)

Plasmid

#72554

Purpose

Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R.

Depositor

Ester Hammond

Article

Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

Insert

p53 (TP53 Human)

Use

Tags

5XHRE

Expression

Mammalian

Mutation

K120R

Promoter

E1B minimal promoter

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAP1892-1

Plasmid

#105247

Purpose

human GFP11 with tagRFP 33bp downstream in Lamin A/C

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

Insertion of GFP11 at cut tagRFP 33bp downstream in Lamin A/C (LMNA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A27

Plasmid

#132004

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A27 (SLC25A27 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC26A10

Plasmid

#131984

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC26A10 (SLC26A10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-tet-puro-sh-ex16

Plasmid

#35167

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHA_RBPMS

Plasmid

#59388

Purpose

Destination vector with RBPMS for stable cell line generation

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

Promoter

CMV, 2x Tet

Available since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC35E4

Plasmid

#132145

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35E4 (SLC35E4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC35F3

Plasmid

#132109

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35F3 (SLC35F3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC35E3

Plasmid

#132062

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35E3 (SLC35E3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC10A5

Plasmid

#131955

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC10A5 (SLC10A5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC35A5

Plasmid

#132111

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35A5 (SLC35A5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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ISG15 C-term. Domain deltaC intein/chitin binding domain (79-154)

Plasmid

#110761

Purpose

Bacterial expression of proISG15

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Use

Tags

intein/chitin binding domain

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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t1-400

Plasmid

#166577

Purpose

For expression of human talin head (residues 1-400) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

Depositor

Vesa Hytönen

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

Insert

Tln1 Head (1-400) (TLN1 Human)

Use

Tags

His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

Expression

Bacterial

Mutation

residues 1-400 only

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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4xnr UAS ifgMosaic Donor vector (IG230)

Plasmid

#99631

Purpose

To clone the 3 ORFs for Gal4 dependent generation of ifgMosaics

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

N-PhiM

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FRT1-His-H2B-Cherry-2A (IG156)

Plasmid

#99622

Purpose

To clone gene of interest downstream of FRT1-His-H2B-Cherry-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

His-H2B-Cherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A24-VN

Plasmid

#135508

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*24:02

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*24:02 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC4A9

Plasmid

#132216

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A9 (SLC4A9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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attB-mCh-Dnmt3b1-Poly(A)-NeoR

Plasmid

#65553

Purpose

Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

mCherry

Expression

Mammalian

Mutation

isoform 1

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pAM4663

Plasmid

#85126

Purpose

NSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.

Depositor

Susan Golden

Article

Chang et al Science. 2015 Jul 17;349(6245):324-8. do

Insert

PkaiB-luciferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAN19-3xFLAG-TIR1-NOSt

Plasmid

#108545

Purpose

Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

Depositor

Keiko Torii

Article

Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

Insert

TRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)

Use

Cloning vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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