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Plasmid#196088PurposeExpresses mouse hnRNPU lacking RGG domain with a 3xFLAG tag in mammalian cellsDepositorInsertSAF-A (Hnrnpu Mouse)
UseTagsN-terminal 3xFLAGExpressionBacterial and MammalianMutationremoved last 154 amino acids on cterminal endPromoterTREAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-LHX9
Plasmid#192921PurposeBarcoded piggybac transposon vector with Dox-inducible expression of LHX9DepositorInsertLHX9 (LHX9 Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-HOXC9
Plasmid#192910PurposeBarcoded piggybac transposon vector with Dox-inducible expression of HOXC9DepositorInsertHOXC9 (HOXC9 Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-LHX1
Plasmid#192905PurposeBarcoded piggybac transposon vector with Dox-inducible expression of LHX1DepositorInsertLHX1 (LHX1 Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-GATA4
Plasmid#192896PurposeBarcoded piggybac transposon vector with Dox-inducible expression of GATA4DepositorInsertGATA4 (GATA4 Human)
UseTagsHAExpressionMammalianMutationCodon-optimizedPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-TRE-V5-TurboID-linker-T2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA
Plasmid#218910PurposePiggyBac transpositionDepositorInsertV5-TurboID-T2A-huGR/BlastR (NR3C1 Human)
UseTagsExpressionMammalianMutation2363G>C and 4155C>T and 4729T>G and 4731…PromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB-Halo,IRES-eGFP
Plasmid#164519PurposeAll-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-CEBPD
Plasmid#192897PurposeBarcoded piggybac transposon vector with Dox-inducible expression of CEBPDDepositorInsertCEBPD (CEBPD Human)
UseTagsExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
miniCRISPRoff-v1
Plasmid#197030PurposeVector encoding a human codon-optimized miniCRISPRoff-v1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoterDepositorInserthumanized miniCRISRPoff-v1
UseTagsExpressionMammalianMutationD52R / T132R / D228A / D406APromoterCBh, CMV, hU6Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA
Plasmid#197026PurposeVector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoterDepositorInserthumanized enOsCas12f1
UseTagsExpressionMammalianMutationD52R / T132RPromoterCBh, CMV, hU6Available sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA
Plasmid#197027PurposeVector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoterDepositorInserthumanized enRhCas12f1
UseTagsExpressionMammalianMutationL270RPromoterCBh, CMV, hU6Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBCAG-rtTAM2-2A-SOX17-GR-IH
Plasmid#165079PurposeUbiquitously expresses rtTAM2, dexamethasone-inducible human SOX17, and hygromycin-resistance gene.DepositorInsertsUsePiggybac transpositionTagsGlucocorticoid ReceptorExpressionMammalianMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-KLF6
Plasmid#192909PurposeBarcoded piggybac transposon vector with Dox-inducible expression of KLF6DepositorInsertKLF6 (KLF6 Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB-Tet-On-Arl13b-RhoA sensor
Plasmid#189613PurposeTet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensorDepositorInsertArl13b
UseTagsGFP and mScarletExpressionMammalianMutationPromoterTet3GAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-HOPX
Plasmid#192923PurposeBarcoded piggybac transposon vector with Dox-inducible expression of HOPXDepositorInsertHOPX (HOPX Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CuO-V5 CASC3 siRes 1-480 WT
Plasmid#158541PurposeFor PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 1-480DepositorInsertCASC3; BTZ; MLN51 (CASC3 Human)
UseTagsV5ExpressionMammalianMutationAmino acids 1-480, C-terminal deletionPromoterCMVAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-EGR1
Plasmid#192913PurposeBarcoded piggybac transposon vector with Dox-inducible expression of EGR1DepositorInsertEGR1 (EGR1 Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA
Plasmid#197024PurposeVector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoterDepositorInserthumanized OsCas12f1
UseTagsExpressionMammalianMutationPromoterCBh, CMV, hU6Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CuO-V5 CASC3 siRes 1-480 188/218-mut
Plasmid#158542PurposeFor PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 1-480DepositorInsertCASC3; BTZ; MLN51 (CASC3 Human)
UseTagsV5ExpressionMammalianMutationAmino acids 1-480, C-terminal deletion, F188D, W2…PromoterCMVAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CuO-V5 CASC3 siRes 110-480 WT
Plasmid#158543PurposeFor PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 110-480DepositorInsertCASC3; BTZ; MLN51 (CASC3 Human)
UseTagsV5ExpressionMammalianMutationAmino acids 110-480, C-terminal deletionPromoterCMVAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only