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Plasmid#189972PurposePart 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAsDepositorInsertT4 td upstream intron-PABP
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean
Plasmid#24334DepositorInsertH2B
UseCre/LoxTagsGFPExpressionWormMutationmCherry driven by an HSP70 promoterCerulean drive…PromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
Plasmid#207863PurposeAAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNADepositorInsertNpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-AtU6-29
Plasmid#66203Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-mSA2
Plasmid#52319PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
UseTagsFLAG, His, MBP, and TEVExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
915-Plakophilin 2a-Flag
Plasmid#32230DepositorInsertPlakophilin 2a (PKP2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
GW1-Mito-pHRed
Plasmid#31474DepositorInsertCox8-tagged pHRed
UseTags4xCox8ExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOpen-DpnIR
Plasmid#165529PurposeUsed to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)DepositorInsertDpnI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDGE792
Plasmid#167300PurposeVector for plant genome editing containing pRPS5a:GFP-Cas9 expression cassette, Bar (BASTA/PPT) selection marker, and FCY-UPP and Ca-Bs3 counterselction markers, but not sgRNAs.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC119-MCS
Plasmid#58807Purposecontains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 1)
Plasmid#191341PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 1)
UseTagsExpressionMammalianMutationPromoterTRE3GVAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-BN
Plasmid#60518PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA
Plasmid#171380PurposeExpression vector for encoding a human codon-optimized RfxCas13d driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.DepositorInserthumanized RfxCas13d
UseTagsHAExpressionMammalianMutationPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_VHL_WT
Plasmid#81874PurposeGateway Donor vector containing VHL , part of the Target Accelerator Plasmid Collection.DepositorInsertVHL (VHL Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
p246
Plasmid#186183PurposeShotgun Metagenomic Spike-inDepositorInsertsynDNA 246
UseSynthetic Biology; CloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p226
Plasmid#186181PurposeShotgun Metagenomic Spike-inDepositorInsertsynDNA 226
UseSynthetic Biology; CloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p236
Plasmid#186182PurposeShotgun Metagenomic Spike-inDepositorInsertsynDNA 236
UseSynthetic Biology; CloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p266
Plasmid#186185PurposeShotgun Metagenomic Spike-inDepositorInsertsynDNA 266
UseSynthetic Biology; CloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-GPD1
Plasmid#83911Purposestable overexpressionDepositorInsertglycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB402
Plasmid#74796PurposeGateway cloning compatible binary vector for expression of gene by CaMV35S promoter.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only