-
Plasmid#216308PurposeExpresses anti-HER2 H2-18 Light chain in mammalian cells. To be paired with H2-18 heavy chain to form the anti-HER2 H2-18 FabDepositorInsertH2-18 Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG/HA-DCP1
Plasmid#215843PurposeExpression of FLAG/HA-DCP1DepositorInsertHuman DCP1 (DCP1B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-nCas9n
Plasmid#217487PurposeExpression of nuclear nCas9nDepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo-otmyd88
Plasmid#217485PurposeExpression of Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmonDepositorInsertBFP-P2A-otPKRFL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG/HA-p54
Plasmid#215844PurposeExpression of FLAG/HA-p54DepositorInsertHuman p54
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ARL16-His6
Plasmid#216484PurposeUsed for exogenous expression of human ARL16, short form, in mammalian cells. The encoded protein contains a C-terminal, glycine rich linker and His6 tagDepositorInsertADP-ribosylation factor like 16 (ARL16 Human)
UseTagsincludes GSGSGSHHHHHH (His6) tag at C-terminusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/hFGFR3-K650M-V5
Plasmid#214909Purposeexpression of the K650M mutant variant of human FGFR3, which is associated with Severe Achondroplasia with Developmental Delay and Acanthosis Nigricans (SADDAN)DepositorInsertFGFR3 (FGFR3 Human)
UseTagsV5/HisExpressionMammalianMutationK650M substitutionPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-TwinStrep
Plasmid#214988PurposeGateway compatible empty vector (transient mammalian expression) with a C-terminal TwinStrep tagDepositorTypeEmpty backboneUseTagsTwinStrepExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mGCC2-FLAG
Plasmid#214166PurposeTransient expression of GCC2DepositorInsertGCC2 (Gcc2 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Bsd-mEGFP
Plasmid#214369PurposeExpression of the monomeric Green Fluorescent ProteinDepositorInsertmEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo-BFP
Plasmid#214367PurposeExpression of the Blue Fluorescent ProteinDepositorInsertBFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnTS-E1015A
Plasmid#213415PurposeVinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).DepositorInsertVcnTS-E1015A (VCL Synthetic, Chicken)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1015 to alanine (E…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnTS-E1021A
Plasmid#213416PurposeVinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).DepositorInsertVcnTS-E1021A (VCL Synthetic, Chicken)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1021 to alanine (E…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnCS-E1015A
Plasmid#213412PurposeVinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).DepositorInsertVcnCS-E1015A (VCL Synthetic, Chicken)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1015 to alanine (E…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnCS-E1021A
Plasmid#213413PurposeVinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).DepositorInsertVcnCS-E1021A (VCL Synthetic, Chicken)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1021 to alanine (E…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi3-91-NLuc
Plasmid#207150PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI3 (GNAI3 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)KPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoA-91-NLuc
Plasmid#207151PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)GPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoB-91-NLuc
Plasmid#207152PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)GPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
GpACR1-pcDNA3.1-mScarlet
Plasmid#204964PurposeExpression of GpACR1 in fusion with mScarlet in mammalian cellsDepositorInsertGpACR1
UseTagsmScarletExpressionMammalianMutationPromoterCMV (+ enhancer)Available sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+FLAG-KrasnatQ61R
Plasmid#206843PurposeTo express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag.DepositorInsertKras (Kras Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Glutamine 61 to ArgininePromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only