Addgene

We narrowed to 15,057 results for: grna

Showing: 10521 - 10540 of 15057 results

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pENTRdeltaGFPmirGE-DroshaT1.5.5

Plasmid

#61084

Purpose

pENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: recommended as backbone.

Depositor

Patrick Salmon

Article

Myburgh et al Mol Ther Nucleic Acids. 2014 Oct 28;3:e2

Insert

deltaGFP

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBbdCas9G_arr2

Plasmid

#149571

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9G(RBSmut)_arr2

Plasmid

#149578

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLVTH-shNP1

Plasmid

#65062

Purpose

Knockdown of NP1

Depositor

Ramon Trullas

Article

Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

Insert

shNP1 (Nptx1 Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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pSMP-SMYD2_2

Plasmid

#36350

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SMYD2 (SMYD2 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSicoR-TTF1i1

Plasmid

#59620

Purpose

Expression of shRNA against human TTF1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

TTF1 (TTF1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pBbdCas9K(-10TC)_arr2

Plasmid

#149584

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9K_arr2

Plasmid

#149570

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9K(RBSmut)_arr2

Plasmid

#149577

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER-ARF4b

Plasmid

#67498

Purpose

Expression of shRNA targeting human ARF4b

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF4 shRNA (ARF4 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSUPER-ARF5a

Plasmid

#67499

Purpose

Expression of shRNA targeting human ARF5a

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF5 shRNA (ARF5 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSUPER-ARF5b

Plasmid

#67500

Purpose

Expression of shRNA targeting human ARF5b

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF5 shRNA (ARF5 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 SCAF8_isoC_CRISPR resistant

Plasmid

#122466

Purpose

pDONR for Gateway cloning

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF8 isoform C CRISPR resistant (SCAF8 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Synonymous mutations conferring resistance to gRN…

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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nSR100L4

Plasmid

#35174

Depositor

Benjamin Blencowe

Article

Calarco et al Cell. 2009 Sep 4;138(5):898-910.

Insert

MmnSR100

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pLL3.7 Mor2 shRNA

Plasmid

#59298

Purpose

3rd generation lentiviral vector expressing shRNA to mouse Opmr1

Depositor

Ulrike Heberlein

Article

Lasek et al Genes Brain Behav. 2007 Nov;6(8):728-35.

Insert

Mor2 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
GluR2_adRNA_(1,20,6)-GFP

Plasmid

#124619

Purpose

Expresses adRNA (1,20,6) targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

GluR2_adRNA(1,20,6)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSuperRetro mouse Hic-5

Plasmid

#11187

Depositor

Bruce Spiegelman

Article

Drori et al Genes Dev. 2005 Feb 1. 19(3):362-75.

Insert

Hic-5 shRNA (Tgfb1i1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
px330-MRPsg4

Plasmid

#87192

Purpose

disruption of human RMRP gene via CRISPR-Cas9, guide 4

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Insert

human MRP RNA CRISPR guide #4 (RMRP Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSicoR-PTPRKi2

Plasmid

#59611

Purpose

Expression of shRNA against human PTPRK

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PTPRK (PTPRK Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-PTPRKi1

Plasmid

#59610

Purpose

Expression of shRNA against human PTPRK

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PTPRK (PTPRK Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only