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Plasmid#184937PurposeExpresses blue fluorescent protein mBlueberry2 and EGFP in mammalian cellsDepositorInsertmBlueberry2-P2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP PR-D25A
Plasmid#101334PurposepBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic activityDepositorInsertNL4-3 envFS
UseLentiviralTagsExpressionMutationProtease D25APromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4 5HT3 LC IRES GFP
Plasmid#123066PurposechemogeneticsDepositorInsertPSAM4 5HT3 LC IRES GFP (Htr3a Mouse)
UseTagsExpressionMammalianMutationL131G Q139L Y217FPromoterCAGAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mouse PKA-Calpha-mEGFP
Plasmid#45521DepositorInsertPKA catalytic subunit alpha tagged by mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Electra1-P2A-EGFP
Plasmid#184935PurposeExpresses blue fluorescent protein Electra1 and EGFP in mammalian cellsDepositorInsertElectra1-P2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-ADAR2-sesRNA-GFP-W3SL
Plasmid#192069PurposeExpression of ADAR2, sesRNA in mammalian cells, with tTA2 as efRNA and ADAR2 overexpression.DepositorInsertsesRNA for mouse Ctip2
UseAAVTagsExpressionMammalianMutationPromoterCbhAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-PYL1-sfGFP-Emerin
Plasmid#121532Purposeexpression of PYL-SfGFP-Emerin, lentivirus vectorDepositorInsertEmerin (EMD Human)
UseLentiviralTagsPYL1-SFGFPExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Akt-FoxO3a-KTR-EGFP
Plasmid#125131PurposeFluorescent reporter for Akt activityDepositorInsertFOXO2 (FOXO3 Human)
UseTagsEnhanced green fluorescent protein (EGFP)ExpressionMammalianMutationFoxO3A 1-402 amino acidPromoterCAG promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 CMV mCherry-eGFP BGH
Plasmid#92280PurposeControl FRET construct; tandem mCherry-eGFP fusionDepositorInsertmCherry-eGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21
Plasmid#138295PurposegRNA for CRISPR/Cas9 knockout of human TRIM21DepositorInsertgRNA TRIM21 (TRIM21 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEVI(408)-ColE-iLight-msfGFP
Plasmid#170268PurposeExpresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.DepositorInsertiLight
UseTagsmsfGFPExpressionBacterialMutationTo design iLight for expression in bacteria, a fu…PromoterpJ23116Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CASI-Cas9C-P2A-turboGFP
Plasmid#80942PurposeExpresses Cas9C in mammalian cells; co-expresses turboGFP.DepositorInsertCas9C
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-hygro-EGFP-DFCP1
Plasmid#174651PurposeVector for overexpression of EGFP-tagged DFCP1 with IRES for hygromycin selection.DepositorInsertEGFP-DFCP1 (ZFYVE1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP Δrev
Plasmid#101348PurposeAll mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.DepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta revPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP IN-D116A
Plasmid#101333PurposepBS NL4-3 envFS eGFP with mutation that disrupts IN catalytic activityDepositorInsertNL4-3 envFS
UseLentiviralTagsExpressionMutationIntegrase D116APromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVH-EF1a-GFP-P2A-MYC
Plasmid#130695PurposeLentiviral plasmids encoding human MYC with co-expressoin of GFP mediated by P2A.DepositorInserthuman MYC (MYC Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1_6xHis-TEV-eGFP-P62
Plasmid#190929PurposePlasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390DepositorInsertP62 (SQSTM1 Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP/CALR wt-FLAG
Plasmid#204525PurposeRetroviral expression of human CALR wt-FLAGDepositorInsertCALR wt-FLAG (CALR Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9-T2A-GFP
Plasmid#53191PurposeCo-expresses human optimized S. pyogenes dCas9 and GFPDepositorInserthumanized dead Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterhUbCAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)
Plasmid#181745Purposedual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1DepositorInserthuman/zebrafish codon optimized SpCas9
UseIn vitro transcription; t7 promoterTagsNLS(SV40)-3xFLAGExpressionBacterialMutationPromoterT7Available sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only