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GFP1-10-miniCMV-GFP11×11-GFP-tDeg

Plasmid

#185404

Purpose

Expresses GFP1-10 and GFP11×11-GFP-tDeg

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

GFP1-10

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3

Plasmid

#87906

Purpose

sgRNA targeting GFP

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgGFP1, sgGFP2, sgGFP3

Use

Tags

Expression

Mammalian

Mutation

Promoter

H1, U6, 7SK

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pFSW GFP IRES GFP

Plasmid

#133815

Purpose

Encodes EGFP that can be used as a control for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Type

Empty backbone

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pGEM-GFP-URA3-GFP

Plasmid

#72606

Purpose

Template for creating a C-terminal GFP tag AND an internal GFP tag from a single transfection of yeast

Depositor

Bruce Granger

Article

Granger et al PLoS One. 2018 Jan 12;13(1):e0191194. do

Inserts

GFP (full)
URA3
GFP (partial)
Optional Linker

Use

Tags

Expression

Mutation

Includes full 819 bp coding sequence of URA3, 435…

Promoter

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAuroraA GFP-AURKA-GFP

Plasmid

#157765

Purpose

Expression of AURKA fused to GFP at both ends in mammalian cells under AurkA promoter

Depositor

Marc Tramier

Article

Bertolin et al ACS Sens. 2019 Aug 23;4(8):2018-2027. do

Insert

AURKA (AURKA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

promoter CMV replaced by AurkA promoter

Promoter

AURKA minimal

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11

Plasmid

#162037

Purpose

Background-free mRNA labeling tripartite superfolder GFP system

Depositor

Hye Yoon Park

Article

Park et al RNA. 2020 Jan;26(1):101-109. doi: 10.126

Insert

GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pNB_EA_R1CiM_4EGFP_R3EGFPi2

Plasmid

#185968

Purpose

Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Molecular IMPLY gate

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-ro1GFP

Plasmid

#82369

Purpose

Expression of redox sensitive GFP in mammalian cells

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro1GFP

Use

Tags

Expression

Mammalian

Mutation

C48S/F64L/T65S/S147C/Q204C

Promoter

CMV

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-ro2GFP

Plasmid

#82370

Purpose

Expression of redox sensitive GFP in mammalian cells

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro2GFP

Use

Tags

Expression

Mammalian

Mutation

C48S/F64L/S147C/Q204C

Promoter

CMV

Available since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mt-ro1GFP

Plasmid

#82407

Purpose

Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro1GFP

Use

Tags

leader sequence of the E1α subunit of pyruvate de…

Expression

Mammalian

Mutation

C48S/F64L/T65S/S147C/Q204C

Promoter

CMV

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

Plasmid

#188541

Purpose

Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

Depositor

Darrell Kotton

Article

Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

Insert

gRNA

Use

CRISPR

Tags

GFP

Expression

Mutation

Promoter

U6

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP_mEGFP-GluA1c

Plasmid

#114136

Purpose

Mammalian expression of the cytosolic C terminus of GluA1 N-terminally tagged by mEGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

mEGFP tagged GluA1 C-terminus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mt-ro2GFP

Plasmid

#82408

Purpose

Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro2GFP

Use

Tags

leader sequence of the E1α subunit of pyruvate de…

Expression

Mammalian

Mutation

C48S/F64L/S147C/Q204C

Promoter

CMV

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcry:GFP,-503unc:hACTA1GFP

Plasmid

#172529

Purpose

alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 503bp unc-45b muscle-specific promoter drives expression of GFP-tagged human ACTA1.

Depositor

Joachim Berger

Article

Berger et al Cell Rep. 2018 Jan 9;22(2):313-322. doi:

Insert

ATCA1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2_-87prox1a:basEGFP_Acry:GFP

Plasmid

#218213

Purpose

Zebrafish -87prox1a enhancer reporter in the lymphatics at 5 dpf. Acry_GFP as a control in the lens

Depositor

Katarzyna Koltowska

Article

Panara et al Development. 2024 May 1;151(9):dev202525

Insert

-87prox1a

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRDAX-491 GFP[1] L3 Stuffer/Intein3 L4 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130174

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-490 GFP[1] L3 Stuffer/Intein2 L4 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130173

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-489 GFP[1] L3 Stuffer/Intein1 L4 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130172

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-488 GFP[1] L6 Stuffer/Intein4 L7 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130171

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-487 GFP[1] L6 Stuffer/Intein3 L7 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130170

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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