-
Plasmid#188248PurposeTemplate plasmid to transcribe mRNA encoding mNeonGreenDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT007-GCN4scFV-VB
Plasmid#163387Purpose2nd generation lentiviral vector to express anti-GCN4 module in the G-baToN systemDepositorInsertantiGCN4scFV-VEEGFRtm-BFP (GCN4scFV-VB)
UseLentiviralTagsBFPExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
WT GLUT1-FLAG
Plasmid#200094PurposeWT human GLUT1 with a C-terminal 3xFLAG tag in pQCXIP vectorDepositorInsertGLUT1 (SLC2A1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-psMEK1tight
Plasmid#89362PurposeA lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cellsDepositorInsertpsMEK1tight (MAP2K1 Synthetic, Human)
UseLentiviralTagsHuman influenza hemagglutinin (HA) tag and pdDron…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-GST
Plasmid#42049DepositorInsertGST
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
hCas9_D10A
Plasmid#41816PurposeExpresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineeringDepositorInsertCas9_D10A
UseCRISPRTagsExpressionMammalianMutationhuman codon-optimized, D10A nickasePromoterCMVAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Cre
Plasmid#24064DepositorInsertCre
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFX-Tom20-mCherry
Plasmid#174188PurposeExpress mCherry with mitochondria-targeting sequence in mammalian cellsDepositorInsertmCherry with mitchondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of TOM20ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT001-LVB
Plasmid#163381PurposeSecond generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN systemDepositorInsertLaG17-VEEGFRtm-BFP (LVB)
UseLentiviralTagsBFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_FLAG-BPTF
Plasmid#102242PurposeExpression of human Flag-BPTF using baculovirus from pFastBac1DepositorInsertFlag-BPTF (BPTF Human)
UseTagsFlagExpressionInsectMutationPromoterPolyhedronAvailable sinceDec. 11, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
BirA in pET28a (w400-2)
Plasmid#26624DepositorInsertBirA
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
Su9-EGFP
Plasmid#23214DepositorInsertSubunit 9 of the F0-ATPase
UseTagsEGFPExpressionMammalianMutationcontains AA 1-69 of subunit 9PromoterAvailable sinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
p5-39
Plasmid#118088PurposeFKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmidDepositorInsertFKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
hyeA3A-BE4max
Plasmid#157944PurposeMammalian expression of hyeA3A-BE4maxDepositorInserthyeA3A-BE4max
UseCRISPRTagsExpressionMammalianMutationN57GPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
OPRD1-Tango
Plasmid#66461PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertOPRD1 (OPRD1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
pDNA2.0 6H-FLAG-TEV-KRAS G12C
Plasmid#111848PurposeExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.DepositorInsertKRAS G12C (KRAS Human)
UseTags6xHis-tag, FLAG-tag, and TEV protease cleavage se…ExpressionBacterialMutationChanged Glycine 12 to CysteinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGTVL2-CRBN-TBD
Plasmid#196369Purposeprotein expression of the GST-tagged Cereblon Thalidomide binding domainDepositorInsertCRBN (CRBN Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationcontains only T319-T425PromoterT7Available sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-Dnmt3a_IM
Plasmid#84478PurposeLentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)DepositorInsertdCas9-Dnmt3a_IM
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
scFLARE1
Plasmid#158738PurposeMammalian expression of scFLARE1 componentDepositorInsertscFLARE1
UseAAVTagsFlag, V5ExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only