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158,707 results

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pLAMP1-miRFP670nano3

Plasmid

#184669

Purpose

LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

LAMP1-miRFP670nano3

Use

Tags

miRFP670nano3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pABE8e-protein

Plasmid

#161788

Purpose

Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

Depositor

David Liu

Article

Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

Insert

ABE8e base editor

Use

Tags

8xHIS and BPNLS

Expression

Bacterial

Mutation

Promoter

pRhaBAD

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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mouse b3 full length

Plasmid

#217814

Purpose

Full length mouse integrin b3 expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGB3 (Itgb3 Mouse)

Use

Tags

P2A-mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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mouse av full length

Plasmid

#217809

Purpose

Full length mouse integrin av expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGAV (Itgav Mouse)

Use

Tags

P2A-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pT3EF1aH-myr-Akt

Plasmid

#179909

Purpose

This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

Depositor

Xin Chen

Article

Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

Insert

Akt1 (Akt1 Mouse)

Use

Tags

HA and Myr

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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UKNP1.16.3

Plasmid

#98188

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4-GFP-Vinculin

Plasmid

#107153

Purpose

mammalian expression

Depositor

Tina Izard

Article

Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

Insert

Vinculin (VCL Human)

Use

Tags

(His)10-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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EC70205

Plasmid

#209466

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas9 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

TaU6

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70203

Plasmid

#209464

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas9 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

TaU6

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Integrin β4-GFP

Plasmid

#205092

Purpose

Express GFP-tagged human integrin β4 in mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

Integrin beta 4 (ITGB4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCAD29_hIRG1_4-461_pvp008

Plasmid

#124843

Purpose

Expresses human CAD (Irg1) in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Use

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 462-end.

Promoter

T7

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-iSAM

Plasmid

#211495

Purpose

AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

Depositor

Lesley Forrester, Antonella Fidanza

Article

Petazzi et al eLife. 2024 Dec 23;13:RP94884. doi: 10.7

Inserts

UniSAM insert
TET-ON
Neo Resistance
Insulator

Use

CRISPR and Synthetic Biology

Tags

T2A-mCherry Tag

Expression

Mammalian

Mutation

Promoter

EF1a, NA, Splicing acceptor, and TREGV promoter

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCS-membrane-ceruleanFP

Plasmid

#53749

Purpose

Contains membrane localized cerulean fluorescent protein in pCS expression vector.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

2x membrane linker

Use

Tags

Cerulean

Expression

Mammalian

Mutation

Promoter

simian CMV IE94

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pDR119

Plasmid

#13332

Depositor

Martin Gellert

Article

Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

Insert

DNA ligase IV (LIG4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
acta2_mCherry_pA_pDestTolpA2

Plasmid

#78685

Purpose

acta2:mCherry transgenesis construct

Depositor

Sarah Childs

Article

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Tags

mCherry

Expression

Mutation

Promoter

acta2

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p246 eSpCas9_2gRNAs_mH11

Plasmid

#164854

Purpose

gRNA vector for targeting mouse H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

gRNAs for targeting mouse H11 locus

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9679-sgRNA-BFP

Plasmid

#162988

Purpose

sgRNA cloning plasmid

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

exchangeable cassette

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRRLsin-SV40 T antigen-IRES-mCherry

Plasmid

#58993

Purpose

lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter

Depositor

Snorri Thorgeirsson

Article

Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

Inserts

CMV enhancer-chicken beta actin promoter
SV40 large T antigen
IRES-mCherry red fluorescent protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

EcoRV site in MCS converted to BamHI site

Promoter

CMV enhancer-chicken beta actin

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR3

Plasmid

#116742

Purpose

Lentiviral expression of FGFR3

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR3 (FGFR3 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-5'dGFP3'

Plasmid

#26425

Depositor

Erin Schuman

Article

Sutton et al Science. 2004 Jun 25. 304(5679):1979-83.

Insert

5'dGFP3'

Use

Tags

CamKIIa 3'-UTR, CamKIIa 5'-UTR, and dGF…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only