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Plasmid#123363PurposeLentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.DepositorInsertEGFP-NLS
UseCRISPR, Lentiviral, and LuciferaseTagsExpressionMammalianMutationPromoterEFSAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBP (YF)
Plasmid#20175DepositorInsertYFP-FKBP (YF): (FKBP1A Human)
UseTagsExpressionMammalianMutationFKBP was taken out from pC4M-F2E with a flanking …PromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro
Plasmid#213030PurposeExpresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2DepositorInsertHuman LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
UseTags3xFlag (ORF2p)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CIB1-mCerulean-MP
Plasmid#58366PurposeFor use in light-induced protein inactivation through the interaction with CRY2DepositorUseTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-enCas12a-2xNLS
Plasmid#209021PurposeLentiviral vector expressing human codon-optimized enCas12a with 2xNLSDepositorInsertenCas12a-2xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationE174R/S542R/K548RPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAM_AraC
Plasmid#91569PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.DepositorInsertAraC/PBAD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9-pAc
Plasmid#162163PurposeExpresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectableDepositorInsertshSpCas9
sgRNA
UseCRISPRTagsT2A-pAcExpressionInsectMutationPromoterAe. aegypti PUb and Ae. aegypti U6Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV
Plasmid#16403PurposeShuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK172-6xHis-0N4R-P301S-tau-HiBiT
Plasmid#187835PurposeExpression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.DepositorInsert6XHis-0N4R-P301S-tau-HiBiT
UseTags6xHistidine and HiBiTExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DHX36iso1
Plasmid#159585PurposeExpress N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cellsDepositorInsertDHX36 (DHX36 Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbS5a-Opto-Cre-Vvd-2
Plasmid#160400PurposeUpdated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.DepositorInsertOpto-Cre-Vvd-2
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-VEGFprom(-1000 to -1)
Plasmid#66128PurposeLuciferase-based reporter for VEGF promoter region (-1000 to -1 bp)DepositorInsertVascular Endothelial Growth Factor -A promoter region (VEGFA Human)
UseLuciferaseTagsExpressionMutationPromoterVEGF -1000 to -1Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3- SREBP-1
Plasmid#204192PurposeMammalian expression of SREBP-1 (isoform 1)DepositorInsertSREBP-1 (SREBF1 Human)
UseTagsE-tag and T7x2ExpressionMammalianMutationmultiple silent mutationsPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-Tspan4
Plasmid#210323PurposeExpresses Tetraspanin-4 (TSPAN4) as migrasome markerDepositorInsertTetraspanin 4 (Tspan4 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-APP-mGFP
Plasmid#196704PurposeExpresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
UseTagsmCherry and mEGFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
ALPaGA
Plasmid#175272PurposeLactate biosensor in anaerobic conditionsDepositorInsertLldR_Synthetic_promoter_sfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUP-(2xCouRS-tRNAopt)
Plasmid#197930PurposeMachinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycineDepositorInsertsCouRS
tRNA opt
UseTagsExpressionBacterialMutationPromoterglnS' and lambda(ts) and proKAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL11024 (pICH47732::NOSp-NPTII-OCST)
Plasmid#51144PurposeConfers KAN resistance in plantaDepositorInsertNOSp-NPTII-OCST
UseTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp-GBP-Tbro
Plasmid#179995PurposeExpresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.DepositorInsertGBP nanobody
UseTagsTrboExpressionInsectMutationPromoterUAS-Gal4Available sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-Sox11
Plasmid#120387Purposeover expression of sox11 using infectionDepositorInsertsox11 (Sox11 Mouse)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only