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Plasmid#200939Purposeendolysosome localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only
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pProEx-IDE-wt
Plasmid#99014PurposeExpress human Insulin Degrading Enzyme (IDE) in E. coli.DepositorInsertinsulin degrading enzyme (IDE Human)
UseTagsHis tagExpressionBacterialMutationPromoterTRCAvailable sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1(nsp8-nsp7)(nsp12)
Plasmid#165451PurposeExpresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia ColiDepositorInsertsnsp12
nsp8
nsp7
UseTags14Histidine-TEVExpressionBacterialMutationPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3b-8XGliBS:EGFP
Plasmid#84602PurposeGli protein-dependent EGFP reporter for analysis of Hedgehog signalingDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoter8X Gli-binding siteAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3Flux (HaloTag7-mGFP-LC3-STOP)
Plasmid#215004PurposeShuttling vector for an autophagy (LC3) reporterDepositorInsertMAP1LC3B (MAP1LC3B Rat)
UseGatewayTagsHaloTag(7)-mGFPExpressionMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCri System
Plasmid Kit#1000000058PurposepCri System: plasmids for cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli, Bacillus subtilis, and Pichia pastoris.DepositorAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD2
Plasmid#20184DepositorInsertNotch 2 intracellular domain (Notch2 Mouse)
UseTags3X FLAGExpressionMammalianMutationContains murine Notch 2 Intracellular Domain (Met…PromoterCMVAvailable sinceJan. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_CEACAM6-His
Plasmid#149337Purposefor eukaryotic expression and purification of CEACAM6-His6DepositorInsertCEACAM6 (CEACAM6 Human)
UseTagsIL-2 signal peptide and polyhistidineExpressionMammalianMutationpoint mutation (GGA to GCA) to replace 'G-g…PromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-MFSD8_STOP
Plasmid#161048PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertMFSD8 (MFSD8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBBR1k-J23101-GFPuv
Plasmid#106388PurposepBBR1k-GFPuv with Anderson promoter J23101 expressing lacIDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Klotho (secreted)
Plasmid#17713DepositorInsertKlotho (KL Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-GFP
Plasmid#28014DepositorInserteGFP
UseAAV; Adeno associate viralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFR3.v857.SGZ
Plasmid#178330PurposeFluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZDepositorInsertpAAV hSyn iGluSnFR3 v857.SGZ
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and Stargazin and PDZ dom…ExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT K842M
Plasmid#154272PurposeBacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationLysine 842 changed to Methionine- bacterial codon…PromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Cytochrome C
Plasmid#41181DepositorInsertCytochrome C (CYCS Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NMHC II-C
Plasmid#10843DepositorInsertNMHC II-C (Myh14 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
p6350 MSCV-CMV-CMV-Flag-HA-ATAD5
Plasmid#31356DepositorInsertATAD5 (ATAD5 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAG1159 TOM20-ECFP-FIREmate
Plasmid#196693PurposeExpresses TOM20-ECFP-FIREmate in mammalian cellsDepositorInsertTOM20-ECFP-FIREmate
UseTagsFIREmateExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange
Plasmid#177827PurposeHost Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)DepositorInsertmOrange
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG415GAL-ccdB
Plasmid#14145DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only