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150,021 results

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pET21b-Caspase-3

Plasmid

#90087

Purpose

Expresses wild type caspase 3 in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

Insert

Caspase 3 (CASP3 Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

Wild type

Promoter

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5 human p27

Plasmid

#14049

Depositor

Joan Massague

Article

Luo et al Nature. 1995 May 11. 375(6527):159-61.

Insert

p27 (CDKN1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

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pBabe-tetoff-mVenus-PICdc42

Plasmid

#91876

Purpose

PI(WT): Photo inhibitable (blue light sensitive)

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Vav2 (VAV2 Human)

Use

Tags

mVenus

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRE57-Ter

Plasmid

#170635

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

Mini-Tn6677, Ter cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp3-His

Plasmid

#11821

Depositor

Guy Salvesen

Article

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 3 (CASP3 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in lentiCRISPR v1

Pooled Library

#1000000100

Purpose

CRISPR gRNA knockout pooled library (version 1) for targeting the human genome

Depositor

Eric Lander, David Sabatini

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only
ER-GCaMP6-210

Plasmid

#86919

Purpose

Fluorescent reporter for ER calcium signaling

Depositor

Timothy Ryan

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

ER-GCaMP6-210

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

Promoter

CAMKII

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-eGFP-PGK-mCherry

Plasmid

#191862

Purpose

Heat inducible eGFP reporter with constitutive mCherry marker

Depositor

Yingxiao Wang

Article

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

eGFP
mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

HSPA7 promoter and PGK promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-MAPKAPK2

Plasmid

#82720

Purpose

Expresses human MAPKAPK2

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MAPKAPK2 (MAPKAPK2 Human)

Use

Tags

3xFLAG and GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pDONR223_ETS1_WT

Plasmid

#82118

Purpose

Gateway Donor vector containing ETS1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ETS1 (ETS1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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hPGK_V5-APEX2-GFP

Plasmid

#105285

Purpose

drives expression of V5-NES-APEX2-GFP from human PGK promotor

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

V5-APEX2-NES

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

human PGK promoter

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCW-GFP-CtIP

Plasmid

#71109

Purpose

mammalian expession of GFP-CtlP

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLminP_Luc2P_RE3

Plasmid

#90337

Purpose

AHR - Aryl hydrocarbon receptor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

AHR

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Drp1-EGFP-StrepII

Plasmid

#174429

Purpose

Expresses human Drp1 Isoform 3 with EGFP at C-terminus in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Use

Tags

EGFP-StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLH

Plasmid

#201671

Purpose

Expression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

Insert

Polymerase eta (POLH Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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p3HA-hMIB1

Plasmid

#33317

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

MIB1 (MIB1 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
pHR CD22-Megf10 CAR

Plasmid

#113016

Purpose

for production of lentivirus encoding CD22 CAR-P Megf10

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD22 CAR-P Megf10 intracellular domain

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VEGFR1-V5/HIS

Plasmid

#205616

Purpose

Expression of human VEGFR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

VEGFR1 receptor tyrosine kinase (FLT1 Human)

Use

Tags

V5-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-hcGAS(157-522)

Plasmid

#108676

Purpose

For expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522

Depositor

Martin Reijns, Andrew Jackson

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

cGAS amino acid 157-522 (CGAS Human)

Use

Tags

GST

Expression

Bacterial

Mutation

contains amino acid 157-522

Promoter

tac

Available since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pTfR-mScarlet-I

Plasmid

#112954

Purpose

In vivo visualization of Transferrin receptors (can be used for colocalization studies)

Depositor

Dorus Gadella

Insert

pTfR

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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