170,273 results

1. mEmerald-Rad21-N-18

   Plasmid

   #54248

   Purpose

   Localization: Centromere, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Rad21 (RAD21 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pMC-6-BeYDV-Rep-RepA-9

   Plasmid

   #197715

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   BeYDV Rep/RepA proteins: native ORFs
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAB061i3

   Plasmid

   #79230

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ; PlacIq lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1; PlacIq

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pK7WG2-mitoTAL1-orf125

   Plasmid

   #158736

   Purpose

   Plant mitoTALEN vector to cut Brassica CMS gene orf125 (orf138)

   Depositor

   Shin-ichi Arimura

   Article

   Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

   Insert

   mitoTALEN left and right to target orf125 (orf138)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP.N1-HDAC6.DC (catalytic deficient)

   Plasmid

   #36189

   Depositor

   Tso-Pang Yao

   Article

   Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

   Insert

   HDAC6.DC (HDAC6 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   H216/611A

   Promoter

   CMV

   Available since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRSET FlipSuc-90u delta1

   Plasmid

   #14475

   Depositor

   Wolf Frommer

   Article

   Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

   Insert

   FlipThuE W283A
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   N192D, W283A, 13 amino acids deleted from linkers

   Promoter

   T7

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSET FlipSuc-90u delta1

   Plasmid

   #14475

   Depositor

   Wolf Frommer

   Article

   Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

   Insert

   FlipThuE W283A
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   N192D, W283A, 13 amino acids deleted from linkers

   Promoter

   T7

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmiCas9

   Plasmid

   #162758

   Purpose

   express miCas9

   Depositor

   Eugene Chen

   Article

   Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

   Insert

   miCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Mammalian

   Mutation

   Contains Brex27 sequence range is 4958-5065

   Promoter

   CMV

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMC-1-BeYDV-LS-L-11

   Plasmid

   #197717

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   BeYDV LSL in c-sense with GG expansion to v-sense ORF position
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMC-1-BeYDV-LRS-L-11

    Plasmid

    #197718

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    BeYDV LRSL in c-sense with GG expansion to v-sense ORF position
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pD649-HAsp-GAS6-COMP5AP-AviTag-9xHis

    Plasmid

    #157511

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    GAS6 (GAS6 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTKEI-Mal-B2

    Plasmid

    #79756

    Purpose

    Single fluorescent protein biosensor for maltose

    Depositor

    David Savage

    Article

    Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

    Insert

    Mal-B2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)

    Plasmid

    #191389

    Purpose

    Donor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hCdt1(1-100)
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSC101-GFPmut2-mScarlet-I TAA

    Plasmid

    #208187

    Purpose

    nonsense mistranslation, TAA stop

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Tags

    Expression

    Bacterial

    Mutation

    Insertion 6TAA*(stop) 7AGT(Ser)

    Promoter

    Ptet+dnaK P1

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPIP_Peptide_Cloning_Vector

    Plasmid

    #172110

    Purpose

    Lentiviral plasmid for protein / peptide overexpression

    Depositor

    Prashant Mali

    Article

    Ford et al Cell Syst. 2021 Jul 21;12(7):716-732.e7.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6 SgRNA RBM20

    Plasmid

    #162735

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    Spacer sequence for RBM20
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pALS3-Ma-sfGFP-TAG150

    Plasmid

    #212121

    Purpose

    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    araC and lpp

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pALS3-Ma-sfGFP-WT

    Plasmid

    #212119

    Purpose

    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    araC and lpp

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBK-Ma pylRS nitroY/haloY-F5

    Plasmid

    #212125

    Purpose

    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.

    Depositor

    Ryan Mehl

    Insert

    M. alvus PylRS 3-NY/HaloY F5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    CTG125CTT, N166A V168S, A223C, W239R

    Promoter

    GlnS (constitutive)

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTKEI-Tre-C04

    Plasmid

    #79754

    Purpose

    Single fluorescent protein biosensor for trehalose

    Depositor

    David Savage

    Article

    Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

    Insert

    Tre-C04
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge