168,273 results

1. pCAG-3xFLAG-BioID2-MCS

   Plasmid

   #186811

   Purpose

   CAG promoter-driven expression of BioID2-fused protein (N terminus)

   Depositor

   Masahito Ikawa

   Article

   Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

   Type

   Empty backbone

   Use

   Tags

   3xFLAG-BioID2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-Atg14L

   Plasmid

   #21635

   Depositor

   Tamotsu Yoshimori

   Article

   Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

   Insert

   Atg14L (ATG14 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH-nCas9-PBE

   Plasmid

   #98163

   Purpose

   Expresses CRISPR base editor in rice

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-nCas9-UGI-NLS
   

   Use

   Rice

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A)

   Plasmid

   #84777

   Purpose

   Second generation lenti expressing Mash1 and Neurogenin for iN transdifferentation to neurons

   Depositor

   Fred Gage

   Article

   Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

   Insert

   Ngn2 and Mash1 with a T2A (NEUROG2, ASCL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   pTIGHT

   Available since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. LZRS-DNMT1

   Plasmid

   #24952

   Depositor

   Paul Khavari

   Article

   Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

   Insert

   DNMT1 (DNMT1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYC1640

   Plasmid

   #158719

   Purpose

   CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pY094

   Plasmid

   #84743

   Purpose

   Expresses huAsCpf1-T2A-GFP and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huAsCpf1
   GFP
   

   Use

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223-MERTK

   Plasmid

   #23900

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   MERTK (MERTK Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. hCXCR4-mTQ2

   Plasmid

   #110196

   Purpose

   Encodes for human CXCR4 coding sequence tagged with the mTQ2 FP

   Depositor

   Sergi Padilla Parra

   Article

   Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

   Insert

   hCXCR4 (CXCR4 Human)
   

   Use

   Tags

   mTurquoise (mTQ2)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. RET-WT

    Plasmid

    #125252

    Purpose

    Expresses human RET in mammalian cells

    Depositor

    Sissy Jhiang

    Article

    Xing et al Endocrinology. 1996 May;137(5):1512-9. d

    Insert

    RET (RET Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAB061i

    Plasmid

    #79226

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ-HA4; PlacIq lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppro1; PlacIq

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Reporter-2

    Plasmid

    #180219

    Purpose

    Surrogate reporter plasmid for the assay of targeted RNA editing

    Depositor

    Wensheng Wei

    Article

    Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

    Insert

    Surrogate reporter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAB076i3

    Plasmid

    #79198

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAJE-Ma pylRS nitroY/haloY-F5

    Plasmid

    #225684

    Purpose

    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.

    Depositor

    Ryan Mehl

    Inserts

    M. alvus PylRS 3-NY/HaloY F5
    M. alvus Pyl-tRNA(6)
    

    Use

    Methanomethylophilus alvus

    Tags

    Expression

    Mutation

    CTG125CTT, N166A, V168S, A223C, W239R

    Promoter

    GlnS and Lpp

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJJB302

    Plasmid

    #107694

    Purpose

    OsActin Promoter-LwaCas13a-HSP Terminator

    Depositor

    Daniel Voytas

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    Cas13a
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsActin

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEmerald-Rad21-N-18

    Plasmid

    #54248

    Purpose

    Localization: Centromere, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Rad21 (RAD21 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pMC-6-BeYDV-Rep-RepA-9

    Plasmid

    #197715

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    BeYDV Rep/RepA proteins: native ORFs
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAB061i3

    Plasmid

    #79230

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ; PlacIq lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppro1; PlacIq

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pK7WG2-mitoTAL1-orf125

    Plasmid

    #158736

    Purpose

    Plant mitoTALEN vector to cut Brassica CMS gene orf125 (orf138)

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    mitoTALEN left and right to target orf125 (orf138)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP.N1-HDAC6.DC (catalytic deficient)

    Plasmid

    #36189

    Depositor

    Tso-Pang Yao

    Article

    Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

    Insert

    HDAC6.DC (HDAC6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    H216/611A

    Promoter

    CMV

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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