-
Plasmid#165573PurposeDNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.DepositorInsertphi29 DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PB-CAG-mRuby3-Gal8-P2A-Zeo
Plasmid#150815PurposeMammalian expression of mRuby3-Galectin8 (Gal8) fusion proteinDepositorInsertmRuby3-Gal8-P2A-Zeo (LGALS8 Synthetic, Human)
UseTagsGal8 is fused to the c-terminus of mRuby3ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Con/Fon EYFP (AAV Retrograde)
Viral Prep#55650-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn Con/Fon EYFP (#55650). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon EYFP plasmid DNA. Synapsin driven, Cre and Flp-dependent EYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFP (Cre- and Flp-dependent)Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-ChRmine-mScarlet (AAV8)
Viral Prep#137158-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-ChRmine-mScarlet (#137158). In addition to the viral particles, you will also receive purified pAAV-nEF-ChRmine-mScarlet plasmid DNA. nEF-driven expression of ChRmine-mScarlet. These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsmScarletAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF1054
Plasmid#142720PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPLAGL1 (PLAGL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TL028_AAV_hSyn:HaloTag-GluA1
Plasmid#192520PurposeExpression of AMPA GluA1 with N terminal HaloTag fusionDepositorInsertHaloTag-GluA1
UseAAV and Cre/LoxTagsHaloTagExpressionMutationPromoterhSynAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (AAV Retrograde)
Viral Prep#162380-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (#162380). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)
Plasmid#163059Purposemito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)DepositorInsertmito-roGFP2-Orp1
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)
Plasmid#197510PurposeCbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)DepositorInsertAAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
UseAAV and CRISPRTagsBPNLS and NpuN(intein)-BPNLSExpressionMutationABE8e mutations in TadA and nSpRY(D10A/A61R)PromoterCbhAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2546
Plasmid#144209PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCEBPA (CEBPA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG_smFP HA
Plasmid#59759Purposeencodes multiple copies of an epitope tag HADepositorInsertsmFP _HA
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGR481
Plasmid#213783PurposeIntegrates onto p1 a catalytically dead TRP5 and URA3 that can be used for selection of transversion mutationsDepositorInsertura3*
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
eGFP STAT1 Y701F
Plasmid#12302DepositorInsertSTAT1 Y701F (STAT1 Human)
UseTagseGFPExpressionMammalianMutationY701FPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
mScarletI-Rab7
Plasmid#112955PurposeIn vivo visualization of Rab7 (can be used for colocalization studies)DepositorArticleInsertRab7
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
nls-mCherry
Plasmid#108881PurposeExpression of nuclear localized mCherryDepositorInsertnls-mCherry
UseCan be used for mammalian expression or in vitro …TagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST_116_LVL2 cam
Plasmid#179332PurposeNT-CRISPR plasmid for a single gRNA.DepositorInsertPtac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKD237-3a-2
Plasmid#90957PurposeExpresses ThsR and mCherry constitutively and sfGFP under the PphsA promoterDepositorInsertsThiosulfate response regulator
Superfolder GFP
mCherry
UseTagsExpressionBacterialMutationPromoterBba_J23100, Bba_J23105, and PphsAAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pLKO.1 Puro shRNA Scramble
Plasmid#162011PurposeLentiviral negative control vector containing scramble shRNADepositorInsertscramble shRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -