-
Plasmid#187883PurposeGal4/UAS sgRNA expression targeting axedDepositorInsert4 sgRNAs targeting axed
UseTagsExpressionMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-4x(tRNA::hiw{sgRNA})
Plasmid#187884PurposeGal4/UAS sgRNA expression targeting hiwDepositorInsert4 sgRNAs targeting hiw
UseTagsExpressionMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-4x(tRNA::dsarm{sgRNA})
Plasmid#187885PurposeGal4/UAS sgRNA expression targeting dsarmDepositorInsert4 sgRNAs targeting dSarm
UseTagsExpressionMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(5A)
Plasmid#192439PurposeEncodes mCherry-tagged Rheb lacking residues 38-42.DepositorInsertmCherry-Rheb(C181S) (RHEB Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb amino acids 38-42 deleted.PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ
Plasmid#192399PurposeOff state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.DepositorInsertAct2::FRT-Rluc-FRT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZ
Plasmid#192398PurposeOn state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.DepositorInsertAct2::FRT-Rluc-FRT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUC
Plasmid#192373PurposeTo test for the repressive potential of the OCS and Transcription Block (TB) fusion with the FRT sites around it.DepositorInsertAct2::FRT-OCS-TB-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC
Plasmid#192376PurposeTo test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.DepositorInsertAct2::FRT-uORF-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ
Plasmid#192375PurposeTo test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.DepositorInsertAct2::FRT-35Ster-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP729_L2_pV01_0I_FRT-OCS-rLUC_TCTP-fLUC
Plasmid#192372PurposeTo test for the repressive potential of the OCS with the FRT sites around it. Then used as a control to represent the off state of many cicuits.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ
Plasmid#192374PurposeTo test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.DepositorInsertAct2::FRT-OCStrunc-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ
Plasmid#192404PurposeOn state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.DepositorInsertB3RT-Act2-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ
Plasmid#192401PurposeOff state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.DepositorInsertAct2::B3RT-Rluc-B3RT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ
Plasmid#192400PurposeOn state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.DepositorInsertAct2::B3RT-Rluc-B3RT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ
Plasmid#192402PurposeOn state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.DepositorInsertFRT-Act2-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ
Plasmid#192405PurposeOff state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.DepositorInsertB3RT-Act2-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ
Plasmid#192403PurposeOff state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.DepositorInsertFRT-Act2-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR-MRE16+MRE153-mut
Plasmid#178910Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseTagsExpressionMutationCTG>AGT and TGC>CAT mir16+153 mutatedPromoterSV40 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2d (FRRG/AAAA)-TS
Plasmid#190037PurposeExpression of wipi2d mutantDepositorInsertWIPI2d (WIPI2 Human)
UseTagsTEV-2xStrep tagExpressionMammalianMutation223FRRG to AAAAPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.040
Plasmid#184974PurposeTest effect of extending a1/a2 on ADE2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2
UseTagsExpressionYeastMutationADE2 donor P272stop, a1/a2 length extended to 27 …PromoterGal7Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only