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Plasmid#146872PurposeBacterial Expression of DmGW182_862-1384-del1004-1115DepositorInsertDmGW182_862-1384-del1004-1115 (gw Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182_862-1384-F961A_L
Plasmid#146824PurposeInsect Expression of DmGW182_862-1384-F961ADepositorInsertDmGW182_862-1384-F961A (gw Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-delCterm-delDUF-dsRNAres_L
Plasmid#146827PurposeInsect Expression of DmGW182-delCterm-delDUF-dsRNAresDepositorInsertDmGW182-delCterm-delDUF-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-DUF-Cterm_L
Plasmid#146832PurposeInsect Expression of DmGW182-DUF-CtermDepositorInsertDmGW182-DUF-Cterm (gw Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-DUF-RBD_L
Plasmid#146833PurposeInsect Expression of DmGW182-DUF-RBDDepositorInsertDmGW182-DUF-RBD (gw Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6A_1-1208-siRNAres_K
Plasmid#146741PurposeMammalian Expression of HsTNRC6A_1-1208-siRNAresDepositorInsertHsTNRC6A_1-1208-siRNAres (TNRC6A Human)
UseTagsExpressionMammalianMutation254-1962 truncated version of the sequence given …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6A-F1359A-siRNAres_K
Plasmid#146744PurposeMammalian Expression of HsTNRC6A-F1359A-siRNAresDepositorInsertHsTNRC6A-F1359A-siRNAres (TNRC6A Human)
UseTagsExpressionMammalianMutationone non silent mutation K1578E compared to the se…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOAD eIF4G1 (1-341)
Plasmid#196833PurposeExpesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.DepositorInserteIF4G1 (TIF4631 Budding Yeast)
UseTagsGal4ExpressionYeastMutationamino acids 1-341PromoterADHAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOBD2 Pab1 WT (123-204)
Plasmid#196834PurposeExpresses Pab1 RRM2 fragment fused to GAL4 DNA binding domainDepositorInsertPab1 (PAB1 Budding Yeast)
UseTagsGal4ExpressionYeastMutation123-204 amino acidsPromoterADHAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOBD2 Pab1 E181R (123-204)
Plasmid#196835PurposeExpresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domainDepositorInsertPab1 (PAB1 Budding Yeast)
UseTagsGal4ExpressionYeastMutation123-204 amino acids & E181RPromoterADHAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-NHERF1 3xSA
Plasmid#195207PurposeMammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphorylation-deficient mutant (S162/339/340A)DepositorInsertNHERF1 (NHERF1 Human)
UseTagsExpressionMammalianMutationS162/339/340APromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-NHERF1 3xSD
Plasmid#195208PurposeMammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphomimetic mutant (S162/339/340D)DepositorInsertNHERF1 (NHERF1 Human)
UseTagsExpressionMammalianMutationS162/339/340DPromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-NHERF1 PDZ1/2*
Plasmid#195211PurposeMammalian expression vector for GFP tagged NHERF1. Dual PDZ1/2 GYGF>GYAA motif mutantDepositorInsertNHERF1 (NHERF1 Human)
UseTagsExpressionMammalianMutationDual PDZ1/2 GYGF>GYAA motif mutantPromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV R221C SPOP 28-359
Plasmid#194116PurposeR221C His SUMO SPOP expression plasmidDepositorInsertSPOP R221C (SPOP Human)
UseTags6His-SUMO-TEVExpressionBacterialMutationR221CPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
PH-miRFP-FRBx2
Plasmid#193569PurposeExpresses PH domain from PLC delta1 tagged with miRFP703 and two FRB mutants (T2098L) in mammalian cells.DepositorInsertPH domain from PLC delta1 (Plcd1 Rat)
UseTagsFRB (T2098L) and miRFP703ExpressionMammalianMutationPH domainPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PH-mCherry-FRBx2
Plasmid#193570PurposeExpresses PH domain from PLC delta1 tagged with mCherry and two FRB mutants (T2098L) in mammalian cells.DepositorInsertPH domain from PLC delta1 (Plcd1 Rat)
UseTagsFRB (T2098L) and mCherryExpressionMammalianMutationPH domainPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mVenusC-14-HP1b Chromo W42A
Plasmid#191399PurposeW42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenusDepositorInsertFLAG-mVenusC-14-HP1b Chromo W42A
UseTags3xFLAGExpressionMammalianMutationW42APromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T564A
Plasmid#191978PurposeTo produce mutants mimicking dephosphorylated Mfn1DepositorInsertMfn1 T564A (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT564APromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T564D
Plasmid#191979PurposeTo produce mutants mimicking phosphorylated Mfn1DepositorInsertMfn1 T564D (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT564DPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-GFP::NMN-Deamidase{dead}
Plasmid#187869PurposeGal4/UAS expression of GFP::NMN-Deamidase(dead)DepositorInsertNMN-Deamidase{dead}
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only