pOBD2 Pab1 E181R (123-204)
(Plasmid
#196835)
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PurposeExpresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domain
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196835 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepOBD2
- Backbone size w/o insert (bp) 7167
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePab1
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)241
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Mutation123-204 amino acids & E181R
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Entrez GenePAB1 (a.k.a. YER165W)
- Promoter ADH
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Tag
/ Fusion Protein
- Gal4 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pOBD2 + NcoI-PAB1(369-612 bp)-SalI. Expresses Pab1 RRM2 fragment with E181R mutation. Expresses Pab1 fragment fused to GAL4BD.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOBD2 Pab1 E181R (123-204) was a gift from Stan Fields (Addgene plasmid # 196835 ; http://n2t.net/addgene:196835 ; RRID:Addgene_196835) -
For your References section:
Combining natural sequence variation with high throughput mutational data to reveal protein interaction sites. Melamed D, Young DL, Miller CR, Fields S. PLoS Genet. 2015 Feb 11;11(2):e1004918. doi: 10.1371/journal.pgen.1004918. eCollection 2015 Feb. 10.1371/journal.pgen.1004918 PubMed 25671604
