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Plasmid#74710PurposeExpresses dCas9-2xNLS-EGFP in mammalian cellsDepositorInsertdCas9-2xNLS-EGFP
UseCRISPRTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
AbVec1.1-IGLC2-XhoI
Plasmid#99575PurposeExpression of immunoglobulin light chains in mammalian cells, human lambda 2 constant regionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas9CyMutL
Plasmid#166981PurposeEncodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repairDepositorInsertsUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationE32KPromoterpCymRC and pLacIAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHW522 (Prab-3::cGAL-C::let-858 3'UTR)
Plasmid#107130PurposecGAL-C driver under the control of C. elegans rab-3 promoterDepositorInsertNLS::gp41-1-C-intein::cGAL(AD)
UseTagsExpressionWormMutationPromoterC. elegans rab-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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8xNFAT-ZsG-hCD8
Plasmid#153417PurposeNFAT-driven ZsGreen-1 reporter gene with human CD8ab geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x2
Plasmid#58772PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-Stuffer
Plasmid#106248PurposeDestination vector for U6::gRNA expression cassetteDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
mRFP-Rab7
Plasmid#14436PurposeMammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.DepositorInsertRab 7 (RAB7A Canis familialis)
UseTagsmRFPExpressionMammalianMutation3 silent mutations near the C-terminusPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cas9-NRCH
Plasmid#136926PurposeExpresses Cas9-NRCH in mammalian cellsDepositorInsertCas9-NRCH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
2MLS-HyperNova/pcDNA3
Plasmid#221457PurposeExpressing HyperNova in mitochondriaDepositorInsertHyperNova fused with a tandem repeat of the mitochondria localization signal from cytochrome c oxidase subunit VIII
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB
Plasmid#110820PurposeConventional dCas9 repressor-dCas9-KRABDepositorInsertdCas9-KRAB
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PML-3/pSG5
Plasmid#50939Purposeexpression of PML with Sumo sites destroyedDepositorInsertPML-3 (PML Human)
UseTagsHAExpressionMammalianMutationK65R, K160R, K490R (Sumo modification sites)PromoterSV40Available sinceJan. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_Cas9-MS2-BB_BbsI
Plasmid#164802PurposeREDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFPDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-rM3D(Gs)-mCherry (AAV8)
Viral Prep#50458-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-DIO-rM3D(Gs)-mCherry (#50458). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-rM3D(Gs)-mCherry plasmid DNA. hSyn-driven, Cre-dependent rM3D(Gs) receptor with an mCherry reporter for chemogenetic excitation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pESC-leu2d(empty)
Plasmid#20120DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCyterm-mScarlet3_N1
Plasmid#189778PurposeConstruct for testing oligimerization of mScarlet3 in mammalian cells by OSER assayDepositorInsertCyterm-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSP6-RNAP
Plasmid#48143PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylADepositorInsertrna polymerase SP6
UseSynthetic BiologyTagsExpressionBacterialMutationV314A (numbering based on NCBI Sequence ID: NP_85…PromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P1
Plasmid#125034PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertPGK1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNTI821
Plasmid#216630PurposeTet-inducible Cal-ID (V5) for Sleeping Beauty transposon systemDepositorArticleInsertCal-ID
UseSleeping beauty transposon systemTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only