Addgene

We narrowed to 23,084 results for: GFP

Showing: 7641 - 7660 of 23084 results

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pAAV-Syn-Chronos-GFP

Plasmid

#59170

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Syn

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoEGFP-Kan

Plasmid

#44900

Purpose

Yeast tagging vector to create gene fusion with yeast optimized EGFP with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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acGFP NES visual barcode

Plasmid

#158673

Purpose

tagging clonal populations with NES acGFP fluorescent protein. Adding NES to acGFP WC

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

acGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-PdCO-EGFP

Plasmid

#198512

Purpose

Expresses optimized PdCO in frame with EGFP under control of CMV promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

Tags

EGFP and Rho1D4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-7aa-ActA-pEGFP-C1

Plasmid

#177406

Purpose

To express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.

Depositor

David Andrews

Article

Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

Insert

ActA tail anchor (actA Listeria monocytogenes)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-Hs4E-T_AF

Plasmid

#148687

Purpose

Mammalian Expression of Hs4E-T

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4E-T (EIF4ENIF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hSyn1-EGFP

Plasmid

#153205

Purpose

Human synapsin-1 (hSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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Integrin Beta1-mPA-GFP

Plasmid

#186302

Purpose

Expresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoter

Depositor

Pakorn Kanchanawong

Article

Jain et al Nat Protoc. 2022 Jul 27. pii: 10.1038/s4

Insert

Integrin Beta 1 (ITGB1 Human)

Use

Tags

mPA-GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pC4M-F2E-GFP-FKBP

Plasmid

#68058

Purpose

express EGFP-tagged FKBP for heterodimerization with FRB in mammalian cells

Depositor

Richard Youle

Article

Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

Insert

EGFP

Use

Tags

2xFKBP and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b T7pCONS TIR-2 sfGFP

Plasmid

#154468

Purpose

pET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Use

Tags

His-thrombin

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-EGFP-NLS

Plasmid

#165441

Purpose

Expresses nuclear EGFP from CMV promoter

Depositor

Esteban Engel

Article

Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

Insert

EGFP-NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-MCS-P2A-eGFP

Plasmid

#163356

Purpose

Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated GFP marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40

Plasmid

#194692

Purpose

hSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Use

AAV

Tags

mEGFP

Expression

Mutation

Promoter

hSyn1

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHB9-EGFP (Hb9 promoter) [TJ#96]

Plasmid

#16275

Depositor

Thomas Jessell

Article

Wilson et al J Neurosci. 2005 Jun 15. 25(24):5710-9.

Insert

HB9 promoter

Use

Mouse Targeting

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only

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AICSDP-36: AAVS1-mEGFP (PGK)

Plasmid

#114404

Purpose

Homology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP

Depositor

Allen Institute for Cell Science

Insert

AAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)

Use

CRISPR; Donor template

Tags

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

Sept. 4, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA4-TO-Hygromycin-sfGFP-MAP

Plasmid

#44100

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Use

Tags

sfGFP-MAP

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT EGFP-miniAID

Plasmid

#101714

Purpose

For amplification of a EGFP-miniAID for N-terminal tagging

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

EGFP-miniAID

Use

Tags

EGFP-miniAID

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-GFP-CaM-uTEVp

Plasmid

#163028

Purpose

FLiCRE protease for HEK293T expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

GFP-CaM-uTEVp

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

Plasmid

#67981

Purpose

Cas9 activity reporter (control) with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLX304-DCK*-GW-IRES-GFP

Plasmid

#176289

Purpose

Gateway vector for use in generating DCK*-POI fusion reporter

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Insert

Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S74E R104M D133A

Promoter

CMV

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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