Targeting oncoproteins with a positive selection assay for protein degraders.
Koduri V, Duplaquet L, Lampson BL, Wang AC, Sabet AH, Ishoey M, Paulk J, Teng M, Harris IS, Endress JE, Liu X, Dasilva E, Paulo JA, Briggs KJ, Doench JG, Ott CJ, Zhang T, Donovan KA, Fischer ES, Gygi SP, Gray NS, Bradner J, Medin JA, Buhrlage SJ, Oser MG, Kaelin WG Jr
Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6263. doi: 10.1126/sciadv.abd6263. Print 2021 Feb. PubMed Article
Koduri V, Duplaquet L, Lampson BL, Wang AC, Sabet AH, Ishoey M, Paulk J, Teng M, Harris IS, Endress JE, Liu X, Dasilva E, Paulo JA, Briggs KJ, Doench JG, Ott CJ, Zhang T, Donovan KA, Fischer ES, Gygi SP, Gray NS, Bradner J, Medin JA, Buhrlage SJ, Oser MG, Kaelin WG Jr
Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6263. doi: 10.1126/sciadv.abd6263. Print 2021 Feb. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176288 | pLX304-IRES-GFP | pLX304 backbone with an IRES-GFP cassette subcloned into the NheI-AgeI sites |
| 176289 | pLX304-DCK*-GW-IRES-GFP | Gateway vector for use in generating DCK*-POI fusion reporter |
| 176291 | pLX304-GW-DCK*-IRES-GFP | Gateway vector for use in generating POI-DCK* fusion reporter |
| 176296 | pLX304-IRES-Td | pLX304 backbone with an IRES-Tdtomato cassette subcloned into the NheI-AgeI sites |
| 176297 | pLX304-DCK*-IRES-GFP | Expresses DCK* and GFP from the same transcript |
| 176298 | pLX304-DCK*-IRES-Td | Expresses DCK* and TdTomato from the same transcript |