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Showing: 741 - 760 of 2510 results

  1. pBlue-hFX(T133A)-HA

    Plasmid
    #14989
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    changed threonine 133 to alanine
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  2. pBlue-hFX(T133E)-HA

    Plasmid
    #14990
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    changed threonine 133 to glutamic acid
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  3. pBlue-hFX-HA(AscI)

    Plasmid
    #15404
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    700 bp insert can be released by digestion with r…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
    15404-plasmid-map-sequence-id-275749

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  4. pHybr_r2a>m2a(silent)-srt-his

    Plasmid
    #124807
    Purpose
    HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2a (Fc silent)
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    L234A/L235A/N297A
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124807-plasmid-map-sequence-id-251832

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  6. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid
    #162877
    Purpose
    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Insert consists of AA1-170
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162877-plasmid-map-sequence-id-321985

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  7. pHybr_r2a>Fab-srt-his

    Plasmid
    #124810
    Purpose
    HDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    rat IgG2a Hinge-G4S-Sortag-Histag
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial
    Mutation
    Promoter
    pUC
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124810-plasmid-map-sequence-id-243698

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  8. pBlue-hFX-HA(NcoI/EcoRI)

    Plasmid
    #15405
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    insert can be released by digestion with REs NcoI…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
    15405-plasmid-map-sequence-id-203076

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  9. pGL3-basic-hFX-promotor(2kb, distal)

    Plasmid
    #14981
    Depositor
    Michael Ristow
    Insert
    human frataxin promoter fragment (FXN Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    includes distal fragment of human frataxin promot…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  13. pGL3-basic-hFX-promotor(2,5kb)

    Plasmid
    #14980
    Depositor
    Michael Ristow
    Insert
    human frataxin promoter fragment (FXN Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    start codon for human frataxin gene has been muta…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  14. pGL3-basic-hFX-promotor(1,3kb)

    Plasmid
    #14979
    Depositor
    Michael Ristow
    Insert
    human frataxin promoter fragment (FXN Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    start codon for human frataxin gene has been muta…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  15. pBlue-hFX(I154F)(AA88)-HA

    Plasmid
    #15403
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1, HA, and HIS
    Expression
    Bacterial
    Mutation
    contains cDNA coding sequence for amino acids 88-…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  16. pGL3-basic-hFX-promotor(1kb)

    Plasmid
    #14978
    Depositor
    Michael Ristow
    Insert
    human frataxin promoter fragment (FXN Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    start codon for human frataxin gene has been muta…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  17. pET28(a)-hFX(I154F)-HA

    Plasmid
    #14992
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1, HA, and HIS
    Expression
    Bacterial
    Mutation
    contains cDNA coding sequence for amino acids 88-…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  18. pSIREN-RetroQ-BPTF-sh28

    Plasmid
    #73669
    Purpose
    pSIREN-RetroQ vector containing shRNA sequence to BPTF
    Depositor
    Joe Landry
    Insert
    shRNA 28 to BPTF (BPTF, Bptf Mouse, Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 22, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    73669-plasmid-map-sequence-id-138080

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  19. pSIREN-RetroQ-BPTF-sh27

    Plasmid
    #73668
    Purpose
    pSIREN-RetroQ vector containing shRNA sequence to BPTF
    Depositor
    Joe Landry
    Insert
    shRNA #27 to BPTF (BPTF, Bptf Mouse, Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 13, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    73668-plasmid-map-sequence-id-141990

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Showing: 741 - 760 of 2510 results