169,069 results

1. pJAM1.19_PB-CIT-PGK-Blast

   Plasmid

   #205564

   Purpose

   Express human CIT

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   CIT (CIT Human)
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-DIO-ATLASsnFlp (AAV8)

   Viral Prep

   #232352-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-DIO-ATLASsnFlp (#232352). In addition to the viral particles, you will also receive purified pAAV-DIO-ATLASsnFlp plasmid DNA. Syn-driven, ATLASsnFlp expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Don Arnold

   Article

   Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

   Promoter

   Syn

   Available Since

   Nov. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

3. pAXY0002

   Plasmid

   #179835

   Purpose

   binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

   Depositor

   Xiaohan Yang

   Article

   Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

   Type

   Empty backbone

   Use

   Binary vector for plant transformation

   Expression

   Plant

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast

   Plasmid

   #205565

   Purpose

   Express human NEDD4L (delta C2 isoform)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   NEDD4L (NEDD4L Human)
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   delta C2 isoform

   Promoter

   EF1

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCXWB-EBNA1

   Plasmid

   #37624

   Purpose

   Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   EBNA-1 (EBNA-1 human herpesvirus 4)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG CMV Empty Puro

   Plasmid

   #237881

   Purpose

   Empty mammalian expression plasmid for cloning untagged proteins

   Depositor

   Meghan Rego, Addgene Research Program

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 12, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLV-EF1a-IRES-Hygro

   Plasmid

   #85134

   Purpose

   lentiviral backbone

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-LSSmGFP

   Plasmid

   #186543

   Purpose

   LSSmGFP fluorescent protein (ex/em = 400/510 nm): cytosolic expression in mammalian cells

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Expression

   Mammalian

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. rAAV2-retro helper

    Plasmid

    #81070

    Purpose

    retrograde AAV helper vector, carrying rep and modified capsid gene

    Depositor

    Alla Karpova, David Schaffer

    Article

    Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

    Inserts

    rep
    rAAV2-retro cap
    

    Use

    AAV and Unspecified; Helper vector

    Mutation

    Pro2Ala, possibly Asp613Gly and changes made to A…

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1 mCherry

    Plasmid

    #128073

    Purpose

    puro exchanged to mCherry

    Depositor

    Oskar Laur

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    no

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-TRE3G-EGFP

    Plasmid

    #52343

    Purpose

    AAVS1 donor vector for inducible gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. EZ-Tet-pLKO-Hygro

    Plasmid

    #85972

    Purpose

    Tet/Dox inducible shRNA lentivirus with hygromycin selection

    Depositor

    Cindy Miranti

    Article

    Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Promoter

    H1 / TetO

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEB1-mGFPmut2

    Plasmid

    #103980

    Purpose

    Plasmid encoding mGFPmut2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mGFPmut2
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S65A, V68L, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF)

    Pooled Library

    #1000000111

    Purpose

    Genome-wide CRISPR gRNA pooled library for activation of human genes

    Depositor

    John Doench, David Root

    Available Since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pORTMAGE-Ec1

    Plasmid

    #138474

    Purpose

    Enables high-efficiency recombineering in E. coli

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    CspRecT
    mutL E32K
    

    Expression

    Bacterial

    Mutation

    E32K

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. CRY2olig-mCherry

    Plasmid

    #60032

    Purpose

    Expresses a fusion of CRY2PHR E490G with mCherry

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2PHR E490G (CRY2 Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    E490G

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTP1122

    Plasmid

    #104159

    Purpose

    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
    

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. PC1575

    Plasmid

    #232088

    Purpose

    Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.

    Depositor

    Pranam Chatterjee

    Article

    Bhat et al Sci Adv. 2025 Jan 24;11(4):eadr8638. doi

    Insert

    uAb with cloning Esp3I restriction site
    

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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