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222,578 results

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C1-MPAct-mRuby3

Plasmid

#155221

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCri System

Plasmid Kit

#1000000058

Purpose

pCri System: plasmids for cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli, Bacillus subtilis, and Pichia pastoris.

Depositor

F Xavier Gomis-Ruth

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only
pmScarlet-H_C1

Plasmid

#85043

Purpose

Mammalian expression of mScarlet-H

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

mScarlet-H

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pmScarlet-H_C1

Plasmid

#85043

Purpose

Mammalian expression of mScarlet-H

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

mScarlet-H

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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EC70202

Plasmid

#209463

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas9 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

TaU6

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Camk2-NES-Twitch-GR

Plasmid

#173044

Purpose

TnC-based calcium sensor with green-red FRET pair ratiometric readout

Depositor

Yiyang Gong

Article

Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

Insert

NES-Twitch-GR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CamK2

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.tRFP

Plasmid

#57826

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.tRFP

Plasmid

#57826

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pSEZ-1

Plasmid

#51868

Purpose

Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans

Depositor

Meng-Qiu Dong

Article

Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature

Insert

psdhb-1::mtLS-cpYFP

Use

Tags

Expression

Worm

Mutation

Promoter

sdhb-1

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pSEZ-1

Plasmid

#51868

Purpose

Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans

Depositor

Meng-Qiu Dong

Article

Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature

Insert

psdhb-1::mtLS-cpYFP

Use

Tags

Expression

Worm

Mutation

Promoter

sdhb-1

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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N1-p18-EGFP

Plasmid

#42334

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p18 (LAMTOR1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
N1-p18-EGFP

Plasmid

#42334

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p18 (LAMTOR1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG-luc

Plasmid

#21850

Depositor

David Ron

Article

Harding et al Mol Cell. 2000 Nov . 6(5):1099-108.

Insert

ATF4 (Atf4 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

ATF4 5' UTR and AUG fused to Luciferase driv…

Promoter

Available since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG-luc

Plasmid

#21850

Depositor

David Ron

Article

Harding et al Mol Cell. 2000 Nov . 6(5):1099-108.

Insert

ATF4 (Atf4 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

ATF4 5' UTR and AUG fused to Luciferase driv…

Promoter

Available since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pHAGE-PPP2R1A

Plasmid

#116777

Purpose

Lentiviral expression of PPP2R1A

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PPP2R1A (PPP2R1A Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pF141 pAcGFP1 SOD1WT

Plasmid

#26402

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pF141 pAcGFP1 SOD1WT

Plasmid

#26402

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pLX317-MBNL1

Plasmid

#115443

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

MBNL1 (MBNL1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1(pCMV5)

Plasmid

#12148

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 (Foxo1 Mouse)

Use

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 13, 2007

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1(pCMV5)

Plasmid

#12148

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 (Foxo1 Mouse)

Use

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 13, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge