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Plasmid#201584PurposeExpresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cellsDepositorInsertPolymerase kappa (POLK Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-EPSIN 1
Plasmid#22228DepositorInsertEpsin 1 (Epn1 Rat)
UseTagsGFPExpressionBacterial and MammalianMutationplease see depositor comment belowPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSF3-microID
Plasmid#172880Purposeexpresses microID in mammalian cells with the tet-on or tet-off systemDepositorInsertmicroID
UseLuciferase and Retroviral; To be used in a tet-on…TagsmycExpressionMammalianMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterTRE-cmvAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMSCVhyg
Plasmid#134323PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-pMJ915v2
Plasmid#88916PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-R-diLACCO1
Plasmid#208102PurposeControl biosensor for intracellular L-lactateDepositorInserteLACCO2.1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-ER-3
Plasmid#54082PurposeLocalization: Endoplasmic Reticulum, Excitation: 487, Emission: 509DepositorInsertER (CALR Human)
UseTagsmEmeraldExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceJune 30, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSAM2_mCherry_Gata4
Plasmid#72690PurposeDoxycyline regulated expression of Gata4 in mammalian cellsDepositorInsertGata 4 (Gata4 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-mScarlet-SARS-CoV-2-5'UTR
Plasmid#184638PurposeFluorescent reporter for mammalian cells: Expression of mScarlet with SARS-CoV-2 5'UTRDepositorInsertmScarlet
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-miR29b
Plasmid#21121DepositorInsertmiR-29b-1 (MIR29B1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGR596
Plasmid#213787PurposeIntegrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationSee manuscriptPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA FLAG TORC2
Plasmid#22975DepositorInsertTORC2 (CRTC2 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9Pubi-B
Plasmid#66188Purposeexpression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotermaize ubiquitin promoter (Pubi)Available sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Trafficking, mitochondrial, motility
Pooled Library#1000000126PurposeCRISPR gRNA mouse knockout pooled library - Trafficking, mitochondrial, and motilityDepositorAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-BBS7
Plasmid#218715PurposeExpresses N-terminally EGFP-tagged BBS7 in mammalian cellsDepositorInsertBBS7 (BBS7 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBP-SpyCatcher
Plasmid#79053PurposeExpresses maltose binding protein (MBP) SpyCatcher fusionDepositorInsertMBP-SpyCatcher
UseTags6xHis and Maltose binding proteinExpressionBacterialMutationDerived from pDEST14-SpyCatcher (Plasmid #35044)…PromoterT7Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ centrin-RFP
Plasmid#26753DepositorInsertCentrin 4 (cetn4 Frog)
UseReporter for basal bodyTagsmonomeric RFPExpressionMutationPromoterAvailable sinceFeb. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX Src-SH2
Plasmid#46510DepositorInsertSrc (SRC Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 142-252PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMU-ERr
Plasmid#61170PurposeFluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoterDepositorInsertAtWAK2-mCherry-HDEL
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceApril 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-LDh-MT-P2A
Plasmid#214896PurposeExpresses equimolar Lipid Droplet- and Mitochondria- targeted split-FAST fragments for Short-range LD-MT Contact sitesDepositorInsertSPLICSFAST-LDh-MT-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only