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Plasmid#34924PurposeExpresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membraneDepositorInsertLck-GCaMP5G
UseTagsExpressionMammalianMutationPlasmid contains N-terminal 26 amino acid membran…PromoterCMVAvailable sinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
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elavl3:SomaGCaMP7f
Plasmid#158760PurposeCell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.DepositorInsertSomaGCaMP7
UseSynthetic BiologyTagsExpressionMutationPromoterHuC promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1
Plasmid#52504Purposemammalian expression vector of BRCA1 wildtype siRNA resistantDepositorInsertBRCA1 (BRCA1 Human)
UseTagsFlagExpressionMammalianMutationsiRNA resistant sequence: AGTATAATCPromoterCMVAvailable sinceFeb. 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDNA5-FRT/TO-eGFP-TONSL
Plasmid#221012Purposemammalian expression vector of eGFP tagged TONSL siRNA resistantDepositorInsertTONSL (TONSL Human)
UseTagseGFPExpressionMammalianMutationsilent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…PromoterCMVAvailable sinceJune 24, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDNA5-FRT/TO-FLAG-TONSL
Plasmid#221011Purposemammalian expression vector of Flag tagged TONSL siRNA resistantDepositorInsertTONSL (TONSL Human)
UseTagsFlagExpressionMammalianMutationsilent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…PromoterCMVAvailable sinceJune 24, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ai62(TITL-tdT) Flp-in replacement vector
Plasmid#61576PurposeRecombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locusDepositorInsertchromatin insulator flanked TRE-LSL-tdTomato
UseRecombinase-mediated cassette exchange using flp …TagsExpressionMutationPromoterAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgCnr1
Plasmid#209196PurposeMutagenesis of Cnr1DepositorInsertCnr1 (Cnr1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterCMVAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet
Plasmid#137161PurposeIntersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-ChRmine-p2a-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationoScarlet E95DPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag::UbvG08 I44A, deltaGG
Plasmid#74939PurposeUbiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1DepositorInsertUbiquitin
UseTagsFlagExpressionMammalianMutationUbvG08 with I44A mutation and no terminal GlycinesPromoterCMVAvailable sinceJune 30, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX4T1-TEV-HsFbxo7-129-398/Skp1
Plasmid#202592PurposeBacterial expression of HsFbxo7-129-398 and HsSkp1DepositorUseTagsGST, TEVExpressionBacterialMutationaa 129-398 onlyPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-SomaGCaMP7
Plasmid#182942PurposeCre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.DepositorInsertSoma-GCaMP7
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rasgrf2-2A-dCre targeting vector
Plasmid#61573PurposeTarget a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 geneDepositorInsertRasgrf2-2A-dCre (Rasgrf2 Mouse, P1 bacteriophage)
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMarch 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-FLAG-SHLD3
Plasmid#114123PurposeExpresses N-terminally tagged FLAG-SHLD3 in mammalian cellsDepositorInsertSHLD3 (SHLD3 Human)
UseGenomic integration, tetracycline inducibleTagsFLAGExpressionMammalianMutationPromoterCMV/TetO2Available sinceAug. 16, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL-CRISPR.EFS.PAC
Plasmid#57828PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A
Plasmid#52505Purposemammalian expression vector of 53BP1 28A mutantDepositorInsert53BP1 28A (TP53BP1 Human)
UseTagsFlagExpressionMammalianMutationAll 28 SQ/TQ motifs in N-term of 53BP1 have been …PromoterAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMV Marina-T2A-nls-mCherry
Plasmid#74216Purposegenetically-encoded fluorescent voltage indicatorDepositorInsertfluorescent protein voltage sensor
UseTagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-PPO-Venus
Plasmid#139505PurposeAAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.DepositorHas ServiceAAV8 and AAV9Insertparapinopsin
UseAAVTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-FLAG-SHLD1
Plasmid#114115PurposeExpresses N-terminally tagged FLAG-SHLD1 in mammalian cellsDepositorInsertSHLD1 (SHLD1 Human)
UseGenomic integration, tetracycline inducibleTagsFLAGExpressionMammalianMutationPromoterCMV/TetO2Available sinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2plus-mCerulean 156-239-GGGS-TDP-43
Plasmid#162617PurposeAllows for transcription of mCerulean fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assaysDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only