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Plasmid#159987PurposeFor the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmCherry-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mCherry and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-BlaM-Vpr
Plasmid#21950DepositorInsertBlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
3xAP1pGL3 (3xAP-1 in pGL3-basic)
Plasmid#40342DepositorInsert3xAP-1
UseLuciferaseTagsLuciferaseExpressionMammalianMutationContains three canonical AP-1 binding sites (TGAC…PromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP GFP-NBR1
Plasmid#38283DepositorInsertNext to BRCA1 gene 1 protein (NBR1 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTW040
Plasmid#64769PurposeFor integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminatorDepositorInsertCre-EBD78
UseTagsfused to the human Estrogen Binding Domain (EBD),…ExpressionYeastMutationPromoterTDH3Available sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
phH1-gRNA
Plasmid#53186PurposeExpresses the S. pyogenes sgRNA from the H1 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman H1Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA (New MCS)
Plasmid#32530DepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTUB1:OsTIR1-3FLAG, SAG1:CAT
Plasmid#87258PurposeTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.DepositorInsertsTIR1
CAT
UseToxoplasma expressionTags3FLAGExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgSAG1 361 bp and TgTUB1 2717 bpAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUAS-V5-miniTurboID-NES
Plasmid#116905PurposeExpresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.DepositorInsertminiTurboID
UseDrosophila expressionTagsExpressionMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV CAP-B10)
Viral Prep#37825-CAP-B10PurposeReady-to-use AAV CAP-B10 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV were produced with the CAP-B10 serotype, which permits efficient transduction of both inhibitory and excitatory neurons in the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPR220.08
Plasmid#78553PurposePlasmid constitutively expressing phycocyanobilin (PCB) biosynthesis genes and UirS sensor kinase geneDepositorInsertuirS
UseSynthetic BiologyTagsExpressionMutationPromoterJ23108Available sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLv-CMV-MyoD-ER(T)
Plasmid#26809DepositorInsertMyoD-ER(T) (Myod1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a-nanoHER2-SpyTag003
Plasmid#216312PurposeExpresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.DepositorInsertnanoHER2-SpyTag003
UseTagsHis6 tag and SpyTag003ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FuG-B2
Plasmid#67513PurposeThis is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.DepositorInsertFusion Glycoprotein type B2
UseLentiviralTagsFusion protein of Vesicular stomatitis Indiana vi…ExpressionMutationPromoterCAGGSAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pABE8e-protein
Plasmid#161788PurposeRhamnose-inducible expression of 8xHIS-ABE8e in E. coliDepositorInsertABE8e base editor
UseTags8xHIS and BPNLSExpressionBacterialMutationPromoterpRhaBADAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPR219.05
Plasmid#78554PurposePlasmid expressing UirR, response regulator and PcsiR1-regulated GFP outputDepositorInsertuirR
UseSynthetic BiologyTagsExpressionMutationPromoterJ23115Available sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-FLAG-SQSTM1
Plasmid#223715PurposeExpresses Flag-tagged SQSTM1DepositorInsertSQSTM1 (SQSTM1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
spNW25
Plasmid#173484PurposeExpresses spNW25 in Bl21DepositorInsertspNW25
UseTagsHistagExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-H2B Control Plasmid
Plasmid#101124PurposeExpression of SNAP-tagged Histone H2B in mammalian cellsDepositorInserthistone cluster 1 H2B family member j (H2BC11 Human)
UseTagsSNAP-tag (SNAPf)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmCherry-MS2coatProtein
Plasmid#103832PurposeLive cell reader for MS2 loop RNA productionDepositorInsertmCherry-MS2 coat protein (cp )
UseTagsExpressionMammalianMutationMCP: nt 202-240 are missing (13 aa), C242G (A81G)…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only