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Hygro-Cas9 donor

Plasmid

#86883

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

Depositor

Danwei Huangfu

Article

Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pET30a-YedK

Plasmid

#86884

Purpose

Bacterial expression of E. coli SRAP ortholog YedK

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

YedK (yedK Eschericia coli)

Use

Tags

6xHis, HA tag, and S-Tag

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1

Plasmid

#86885

Purpose

C-terminal part of the split-BioID

Depositor

Mathieu Bollen

Article

Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

Insert

BirA* (AA141-321)- HA- NIPP1]

Use

Tags

HA tag

Expression

Mammalian

Mutation

BirA Amino Acids 141-321

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 - PP1g - Flag - BirA* (AA2-140)

Plasmid

#86886

Purpose

N-terminal part of the split-BioID

Depositor

Mathieu Bollen

Article

Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

Insert

PP1g - FLAG - BirA (AA2-140)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

BirA Amino Acids 2-140

Promoter

Availability

Academic Institutions and Nonprofits only

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pET15b-PvuRts1I

Plasmid

#86887

Purpose

express N-term His tagged PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pET28a-PvuRts1I

Plasmid

#86888

Purpose

express C-term His tagged PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Use

Tags

C-term His tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET15b-D70A-PvuRts1I

Plasmid

#86890

Purpose

express D70A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Use

Tags

His

Expression

Bacterial

Mutation

D70A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET15b-R208A-PvuRts1I

Plasmid

#86891

Purpose

express R208A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Use

Tags

His

Expression

Bacterial

Mutation

R208A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET15b-D57A-PvuRts1I

Plasmid

#86892

Purpose

express D57A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Use

Tags

His

Expression

Bacterial

Mutation

D57A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET15b-H74A-PvuRts1I

Plasmid

#86894

Purpose

express H74A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Use

Tags

His

Expression

Bacterial

Mutation

W205A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET15b-E71A-PvuRts1I

Plasmid

#86895

Purpose

express E71A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Use

Tags

His

Expression

Bacterial

Mutation

E71A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET15b-E228A-PvuRts1I

Plasmid

#86896

Purpose

express E228A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Use

Tags

His

Expression

Bacterial

Mutation

E228A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET15b-E20A-PvuRts1I

Plasmid

#86897

Purpose

express E20A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

Pvurts1I (pvuRts1I )

Use

Tags

His

Expression

Bacterial

Mutation

E20A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET15b-W215A-PvuRts1I

Plasmid

#86898

Purpose

express W215A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Use

Tags

His

Expression

Bacterial

Mutation

W215A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pTXB1-AbaSFI

Plasmid

#86900

Purpose

Express CBD and intein tag containing AbaSI enzyme full length

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

AbaSDFI

Use

Tags

CBD

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6-150

Plasmid

#86918

Purpose

Fluorescent reporter for ER calcium signaling

Depositor

Timothy Ryan

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

ER-GCaMP6-150

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

Promoter

CAMKII

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6-210

Plasmid

#86919

Purpose

Fluorescent reporter for ER calcium signaling

Depositor

Timothy Ryan

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

ER-GCaMP6-210

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

Promoter

CAMKII

Availability

Academic Institutions and Nonprofits only

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pXP420 v2 (repaired Amp promoter)

Plasmid

#86920

Purpose

Shuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.

Depositor

Gary Yellen

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-GFP-Ubl4A

Plasmid

#86921

Purpose

To express Ubl4A tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-GFP-Ubl4A-N(1-89)

Plasmid

#86922

Purpose

To express Ubl4A (1-89 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 90-157 is deleted

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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