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Plasmid#129401PurposemCherry mRNA reporter tagged with (F30-2xPepper)10DepositorInsertmCherry-(F30-2xPepper)10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET_BGL2
Plasmid#101911PurposeExpresses a β-glucosidase gene from Thermobaculum terrenum in E. coli under the control of the pET system.DepositorInsertβ-glucosidase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7+LacOAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-HypocratesCS
Plasmid#161960PurposeMammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivativesDepositorInsertHypocrates
UseTagsExpressionMammalianMutationchanged Cysteine 355 to SerinePromoterCMV, SP6Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCRISPaint-T2A-FLPo-3xP3-RFP
Plasmid#127561PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.DepositorInsertFLPo
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Orange Nano-lantern(Ca2+)/pcDNA3
Plasmid#71346PurposeExpress Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cellDepositorInsertOrange Nano-lantern(Ca2+)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDLX-TVA-2A-N2cG
Plasmid#172364PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from inhibitory neuronsDepositorInsertTVA + B19-N2cG
UseAAVTagsExpressionMammalianMutationPromotermDLXAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-mTmod3-WT
Plasmid#70753Purposemouse Tropomodulin3, WT for bacterial expressionDepositorInsertTropomodulin3 (Tmod3 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
EC71022
Plasmid#159608PurposeLevel 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxPDepositorInsertloxP-mCherry-HDEL-t35S-loxP
UseCre/Lox and Synthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWR
Plasmid#112905PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of tdTomato.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3XFLAG-EGFP-NLS hygro
Plasmid#87781PurposeInducible expression of EGFP with a C-terminal NLS sequenceDepositorInserteGFP
UseLentiviralTags3xFLAG and NLSExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pTriEx-NTOM20-LOV2
Plasmid#81009PurposeExpression of LOV2 anchored to mitochondriaDepositorInsertLOV2
UseTagsNTOM20ExpressionBacterial, Insect, and Mamm…MutationPromoterCMV, p10Available sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC35G5_STOP
Plasmid#161274PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC35G5 (SLC35G5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SLC35G6_STOP
Plasmid#161262PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC35G6 (SLC35G6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
VRK1_HUMAN_D0
Plasmid#79684PurposeThis plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertVRK1 (VRK1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
1518_pAAV-U6-SA-eGFP-gRNA-HLP-SACas9-HA-OLLAS-spA
Plasmid#109316PurposePlasmid for liver-specific expression of AAV SaCas9 with a gRNA against eGFPDepositorInserteGFP gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE
Plasmid#60906PurposeThe plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imagingDepositorInsertscFv-GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
sgGFP_3
Plasmid#78165PurposesgGFPDepositorInsertGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only