Showing: 7121 - 7140 of 147582 results
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Plasmid#86495Purposemamalian cell expression of FGFR3-FGFR1 chimeraDepositorUseTagsExpressionMammalianMutationFGFR3-FGFR1 chimeraPromoterMoloney murine leukemia virus LTRAvailabilityAcademic Institutions and Nonprofits only
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MoFGFR31c-ires neo
Plasmid#86496Purposemamalian cell expression of FGFR3-FGFR1 chimeraDepositorUseTagsExpressionMammalianMutationFGFR3-FGFR1 chimeraPromoterMoloney murine leukemia virus LTRAvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH Yap S127A
Plasmid#86497PurposeMammalian expression of FLAG-tagged Yap containing S127A mutationDepositorInsertYes associated protein 1 (YAP1 Human)
UseTagsFLAGExpressionMammalianMutationS127APromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH c-Met
Plasmid#86498PurposeMammalian expression of human c-MetDepositorInsertMet (MET Human)
UseTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH N90-beta-catenin
Plasmid#86499PurposeMammalian expression of Myc tagged N90 beta-cateninDepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsMycExpressionMammalianMutationDeletion of amino acids 1-90; constitutively acti…PromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH NICD1
Plasmid#86500PurposeMammalian expression of Myc tagged NICD1DepositorInsertNotch1 (C-term) (Notch1 Mouse)
UseTagsMycExpressionMammalianMutationCytoplasmic domain only (AA 1753-2531)PromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
CIAR_pcDNA5/FRT/TO
Plasmid#86502PurposeExpresses CIAR in mammalian cells. Used to generate Flp-In stables.DepositorInsertCIAR (SOS1 Human)
UseFrtTagsExpressionMammalianMutationT968L in SOScatPromoterCMV (tet operator)AvailabilityAcademic Institutions and Nonprofits only -
GFP-P2A-CIAR_pLenti_CMV_Puro
Plasmid#86503PurposeLenti plasmid for generating CIAR expressing stable cell linesDepositorInsertGFP-P2A-CIAR (SOS1 Human)
UseLentiviralTagsExpressionMammalianMutationT968L in SOScatPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
xCIAR_pcDNA5/FRT/TO
Plasmid#86504PurposeExpresses inactive control CIAR (xCIAR) in mammalian cells. Used to generate Flp-In stables.DepositorInsertxCIAR (SOS1 Human)
UseFrtTagsExpressionMammalianMutationF929A and T968L in SOScatPromoterCMV (tet operator)AvailabilityAcademic Institutions and Nonprofits only -
CRAF_S259A_pcDNA3.1
Plasmid#86505PurposeExpresses CRAF_S259A in mammalian cellsDepositorInsertCRAF (RAF1 Human)
UseTagsFLAGExpressionMammalianMutationS259APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
CRAF_S29A_pcDNA3.1
Plasmid#86506PurposeExpresses CRAF_S29A in mammalian cells [Edit]DepositorInsertCRAF (RAF1 Human)
UseTagsFLAGExpressionMammalianMutationS29APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pMSCV-ArchT-GFP
Plasmid#86536PurposeRetrovirus expressing optogenetic silencer ArchT-GFPDepositorInsertArchT
UseRetroviralTagsGFPExpressionMammalianMutationPromoterSynapsin I (human)AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-GFP
Plasmid#86537PurposeRetrovirus expressing GFPDepositorInsertGFP
UseRetroviralTagsExpressionMammalianMutationPromoterSynapsin I (human)AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_plasmid_donor_RD
Plasmid#86551PurposeHomology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabainDepositorInsertATP1A1 (ATP1A1 Human)
UseCRISPR and TALEN; Co-selection via hdr using ouab…TagsExpressionMammalianMutationQ118R, N129DPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLinker-6xHA-HXGPRT-LoxP
Plasmid#86552PurposeThe plasmid is used for endogenous tagging using CRISPR technology in ToxoplasmaDepositorInsertLinker-6xHA-HGPRT-LoxP
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLinker-AID-3xHA-HXGPRT-LoxP
Plasmid#86553Purposeused for a TIR1-AID system with CRISPR tagging technology in Toxoplasma gondiiDepositorInsertLinker-AID-3xHA-HXGPRT 3'UTR
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAP84
Plasmid#86554PurposeExpresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficileDepositorInsertPveg-sLucopt
UseLuciferase and Synthetic BiologyTagsExpressionBacterialMutationPromoterPvegAvailabilityAcademic Institutions and Nonprofits only -
pFH6
Plasmid#86555PurposeSubcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiencyDepositorInsertU6-26p::sgRNA scaffold
UseCRISPR; SubcloningTagsExpressionMutationPromoterArabidopsis U6-26 promoterAvailabilityAcademic Institutions and Nonprofits only -
pUB-Cas9
Plasmid#86556PurposeFor ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)DepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationCas9 is codon optimized for C. reinhardtiiPromoterUBIQUITIN10AvailabilityAcademic Institutions and Nonprofits only -
pREPROII-attB
Plasmid#86557PurposeDox inducible expression of reprogramming factors (Klf4, Sox2, Oct4, & cMyc), integration via phiC31 attB sites, excision via Cre/loxPDepositorUseCre/LoxTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 7121 - 7140 of 147582 results