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Plasmid#212707PurposeTornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.DepositorInsertmutTornado-CVB3-split-nLuc
UseTagsExpressionMutationDeleted the partial 3'ribozyme sequence in T…PromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PRB
Plasmid#89130Purposemammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418DepositorInserthuman progesterone receptor-B (PGR Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
NL4-3 mCherry Luciferase
Plasmid#44965PurposeHIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.DepositorInsertmCherry, Luciferase, IRES
UseLentiviralTagsLuciferase, IRES and mCherry with D212GExpressionMutationS34C mutation in NefPromoterAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nluc
Plasmid#180501PurposeMammalian expression of NlucDepositorInsertNluc
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Fis1
Plasmid#182580PurposeExpresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequenceDepositorInsertmCherry-Fis1
UseTagsFis1ExpressionMammalianMutationPromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1
Plasmid#138460Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRABDepositorInsertMCP-KRAB-IRES-zsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-SopF
Plasmid#135174PurposeExpresses an mCherry-SopF fusion in mammalian cellsDepositorInsertSopF (STM1239 Salmonella enterica serovar Typhimurium)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
VinculinTS
Plasmid#26019DepositorInsertVinculin tension sensor
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP-TIS11B
Plasmid#121157PurposeMammalian expression of GFP tagged TIS11BDepositorInsertZFP36L1 (TIS11B) (ZFP36L1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Hs.cargo(Cre)
Plasmid#174863PurposeExpresses human Peg10 cargoRNA encoding Cre recombinaseDepositorInsertCre flanked by HsPeg10 UTRs
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB501
Plasmid#74843PurposeGateway cloning compatible binary vector for simple cloning of gene.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNo PromoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CXCR4
Plasmid#98942PurposeMammalian expression plasmid for untagged human CXCR4DepositorInsertCXCR4 (CXCR4 Human)
UseTagsExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Clover
Plasmid#40259PurposeGFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlapDepositorHas ServiceCloning Grade DNAInsertClover GFP
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
Plasmid#207860PurposeAAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairsDepositorInsertNpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST (AAV9)
Viral Prep#109048-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST (#109048). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST plasmid DNA. Cre-dependent expression of the anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites, translated from an internal ribosomal entry site (IRES) following mRuby3 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmRuby3Available sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)
Plasmid#140002PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lyn11-targeted FRB (LDR)
Plasmid#20147DepositorInsertLyn-linker-FRB (LDR) (MTOR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
AIO-Puro
Plasmid#74630PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRTagsPuromycin resistant markerExpressionMammalianMutationPromoterCbhAvailable sinceMay 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-CAG-hrGFP
Plasmid#52344PurposeAAVS1 donor vector for constitutive gene expressionDepositorInserthrGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AAV5cap
Plasmid#198015PurposeFor high-level expression of the AAV5 cap gene, suitable for use with pCMV-Rep78/68 SSM libraryDepositorInsertAAV5 cap
UseAAVTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only