Comprehensive mutagenesis maps the effect of all single codon mutations in the AAV2 rep gene on AAV production
Jain NK, Ogden PJ, Church GM
bioRxiv 2023.01.31.526541
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
198014 | pCMV-AAV2cap | For high-level expression of the AAV2 cap gene, suitable for use with pCMV-Rep78/68 SSM library |
198015 | pCMV-AAV5cap | For high-level expression of the AAV5 cap gene, suitable for use with pCMV-Rep78/68 SSM library |
198016 | pCMV-AAV9cap | For high-level expression of the AAV9 cap gene, suitable for use with pCMV-Rep78/68 SSM library |
198050 | pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) | By supplying an AAV cap gene of interest in trans, researchers can use this library to investigate the effects of rep mutations on production of a wide range of AAV capsid serotypes |