pCMV-AAV2cap
(Plasmid
#198014)
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PurposeFor high-level expression of the AAV2 cap gene, suitable for use with pCMV-Rep78/68 SSM library
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 198014 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV-LacZ
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Backbone manufacturerTakara
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAAV2 cap
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Speciesadeno-associated virus type 2
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MutationWT
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-AAV2cap was a gift from George Church (Addgene plasmid # 198014 ; http://n2t.net/addgene:198014 ; RRID:Addgene_198014) -
For your References section:
Comprehensive mutagenesis maps the effect of all single codon mutations in the AAV2 rep gene on AAV production. Jain NK, Ogden PJ, Church GM. bioRxiv 2023.01.31.526541 10.1101/2023.01.31.526541