168,515 results

1. pimEJ5GFP

   Plasmid

   #44026

   Depositor

   Jeremy Stark

   Article

   Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

   Insert

   EJ5GFP egfp-based chromosomal break reporter
   

   Expression

   Mammalian

   Mutation

   pCAGGS promoter and eGFP separated by pgkPURO cas…

   Promoter

   pCAGGS

   Available Since

   May 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TRE-dCas9-Zim3-MeCP2

   Plasmid

   #220143

   Purpose

   doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

   Depositor

   Frank Buchholz

   Article

   Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

   Insert

   dCas9-Zim3-MeCP2
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   Tre-3G

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpyCatcher003 S49C

   Plasmid

   #133448

   Purpose

   Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003 S49C
   

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Contains S49C mutation

   Promoter

   T7

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+) Drp1

   Plasmid

   #34706

   Depositor

   David Chan

   Insert

   Drp1 (Dnm1l Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSC-NGN2-IRES-ISL1-T2A-LHX3

   Plasmid

   #221814

   Purpose

   Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.

   Depositor

   Baojin Ding

   Article

   Sepehrimanesh et al Am J Physiol Cell Physiol. 2020 Oct 1;31

   Inserts

   NEUROG2 (NEUROG2 Human)
   ISL1 (ISL1 Human)
   LHX3 (LHX3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. POLG-pcDNA3.1

   Plasmid

   #191487

   Purpose

   Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.

   Depositor

   Hans Zempel

   Insert

   POLG (POLG Human)
   

   Tags

   V5 tag, 6X His

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Sequestosome1-N-18

   Plasmid

   #55132

   Purpose

   Localization: Membrane Scaffold, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Sequestosome1 (SQSTM1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

8. EC70188

   Plasmid

   #209449

   Purpose

   Cloning protospacers for Cas9 editing

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Insert

   Cas9 sgRNA
   

   Use

   CRISPR

   Promoter

   HvU3

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFD3-dU6:3gRNA

   Plasmid

   #49410

   Purpose

   expression of gRNA under control of the Drosophila U6:3 promoter

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   dU6-3:gRNA
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   dU6-3

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. mApple-TfR-RUSH

    Plasmid

    #166906

    Purpose

    RUSH cargo: Str-KDEL_IRES_Transferrin Receptor (TfR)-SBP-mApple

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    mApple
    

    Expression

    Mammalian

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7RNAP

    Plasmid

    #170101

    Purpose

    expresses T7 RNAP

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    T7-RNA polymerase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    p70a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a-Cas9-2XNES-2ERT2

    Plasmid

    #120551

    Purpose

    Express Cas9, 2xNES and 2 tandem ERT2 in mammalian cells

    Depositor

    Yu Wang

    Article

    Zhao et al Mol Ther Nucleic Acids. 2018 Dec 7;13:20

    Insert

    Cas9, NES and ERT2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-ZO1-C-14

    Plasmid

    #55165

    Purpose

    Localization: Tight Junctions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    ZO1 (TJP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_iPE-C_P2A_Csy4

    Plasmid

    #232428

    Purpose

    Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    iPE-C–P2A–Csy4
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

    Plasmid

    #31482

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (Sce Target) EF Puro
    

    Use

    Lentiviral

    Mutation

    mCherry has M9S and M16L mutations. This is what …

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpyDock

    Plasmid

    #124618

    Purpose

    Expresses SpyDock to bind SpyTag non-covalently for affinity purification

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyDock
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E77A mutation prevents isopeptide bond formation,…

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-Ostir-neo

    Plasmid

    #161973

    Purpose

    Expresses Ostir and G418 resistance gene in mammalian cells

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    Ostir-3xHA-p2a-neoR
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA Flag-RARalpha

    Plasmid

    #35555

    Depositor

    Jonathan Kurie, Harish Srinivas

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Tags

    Flag and His

    Expression

    Mammalian

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTreTight-Htt94Q-CFP

    Plasmid

    #23966

    Depositor

    Nico Dantuma

    Article

    Maynard et al Proc Natl Acad Sci U S A. 2009 Aug 18. 1

    Insert

    N-terminal fragment Huntingtin Q94 (HTT Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    94 polyQ repeat

    Available Since

    April 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV enAsCas12f-HKRA-sgRNA_v7

    Plasmid

    #208357

    Purpose

    Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-HKRA
    

    Expression

    Mammalian

    Mutation

    I123H/D195K/D208R/V232A

    Promoter

    CMV

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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