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7xE-Box::Renilla

Plasmid

#124532

Purpose

c-Myc Renilla luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

c-Myc Renilla reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-MBP-2xNLS-tdTomato

Plasmid

#104054

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-tdTomato

Use

AAV

Tags

NLS

Promoter

MBP

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGL4.10/RSV_SF3B3-miniXon_Luciferase

Plasmid

#174660

Purpose

Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.

Depositor

Beverly L. Davidson

Article

Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

Insert

miniXon cassette

Use

Luciferase

Expression

Mammalian

Mutation

Kozak ATG initiation sequence in middle exon

Promoter

RSV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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TetOn-mCherry-eGFP-RAMP4

Plasmid

#109014

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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ZsGreen1-cMYC/pLVX-Puromycin

Plasmid

#180278

Purpose

Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

Depositor

Andre Catic

Article

Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

Insert

ZsGreen1-cMYC fusion protein MYC (MYC Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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hCRISPRa-v2, genome-wide, 10 sgRNAs/gene

Pooled Library

#1000000091

Purpose

Genome-wide CRISPR gRNA pooled library for activation of human genes

Depositor

Jonathan Weissman

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only
eIF2a 1

Plasmid

#21807

Depositor

David Ron

Insert

eIF2a (EIF2S1 Human)

Expression

Mammalian

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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PB-TAC-ERP2-(N-HA)KRAS_G12D

Plasmid

#201254

Purpose

Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

Depositor

Alexander Kleger

Article

Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

Insert

KRAS (KRAS Human)

Use

Piggybac transposon

Tags

HA-Tag

Expression

Mammalian

Mutation

changed Gly (G) 12 to Asp (D)

Promoter

TRE

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCambia1300-PacI

Plasmid

#44183

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Type

Empty backbone

Expression

Bacterial

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only

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pX459_gRNA-AAVS1_hspCas9

Plasmid

#193309

Purpose

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Use

CRISPR

Expression

Mammalian

Mutation

not applicable

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP104-mCherry (AAV5)

Viral Prep

#58909-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV-GFAP104-mCherry (#58909). In addition to the viral particles, you will also receive purified pAAV-GFAP104-mCherry plasmid DNA. GFAP104-driven mCherry expression control. These AAV preparations are suitable purity for injection into animals.

Depositor

Edward Boyden

Article

Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/nc

Promoter

GFAP

Tags

mCherry

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only
pVSV eGFP RABV-G

Plasmid

#31833

Depositor

Connie Cepko

Article

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV genome

Expression

Bacterial

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pJYDN

Plasmid

#162450

Purpose

Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

Aga2p, HA, myc tags, and eUnaG2 fluorescent prote…

Expression

Yeast

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5 bovine GRK2

Plasmid

#14691

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

Insert

GRK2 (GRK2 Bovine)

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pGH125_dCas9-Blast

Plasmid

#85417

Purpose

lentiviral expression vector for dCas9 with Blasticidin selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

dCas9 and Blasticidin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-BFP

Plasmid

#78545

Purpose

Fusion protein Cas9+BFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

BFP

Use

Lentiviral

Expression

Mammalian

Promoter

Fusion protein with Cas9

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-V5-hTEV

Plasmid

#65800

Purpose

expresses human codon-optimised TEV protease

Depositor

Douglas Green

Article

Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

Insert

V5-human TEV

Tags

V5

Expression

Mammalian

Mutation

codon-optimised to express in mammalian cells

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pSMAL-CellTag-V1

Pooled Library

#115643

Purpose

Viral barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics

Depositor

Samantha Morris

Article

Biddy et al Nature. 2018 Dec;564(7735):219-224. doi:

Has Service

Concentrated Lentiviral Prep

Expression

Mammalian

Species

Synthetic

Use

Lentiviral

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only
pFA6a-TurboID-3MYC-kanMX6 (pFB1420)

Plasmid

#126049

Purpose

For C-terminal tagging with TurboID in yeast including geneticin-resistant marker

Depositor

François Bachand

Article

Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

Insert

TurboID

Use

Pcr-based c-terminal tagging

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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