167,948 results

1. mVenus C1

   Plasmid

   #27794

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Has Service

   Cloning Grade DNA

   Insert

   mVenus C1
   

   Expression

   Mammalian

   Available Since

   April 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pA-His6-Tn5

   Plasmid

   #171871

   Purpose

   Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

   Depositor

   Kaiwei Liang

   Article

   Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

   Insert

   Protein A and hyperactive Tn5 transposase
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFD5_w

   Plasmid

   #112645

   Purpose

   Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

   Depositor

   Michael Boutros

   Insert

   U6:3-tRNA-sgRNA expression cassette
   

   Expression

   Insect

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. ER-mNeonGreen

   Plasmid

   #137804

   Purpose

   Visualization of the Endoplasmic Reticulum

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   KDEL
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-GFP (AAV1)

   Viral Prep

   #37825-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   GFP

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

6. pET His6 GST TEV LIC cloning vector (1G)

   Plasmid

   #29655

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, GST, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57-CMV-ssRFP-GFP-KDEL

   Plasmid

   #128257

   Purpose

   Fluorescence reporter to monitor ER-phagy activity

   Depositor

   Noboru Mizushima

   Article

   Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

   Insert

   ssRFPGFPKDEL
   

   Use

   Lentiviral

   Tags

   RFPGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a(+)-Bst LF-6xHis

   Plasmid

   #159148

   Purpose

   expresses His-tagged large fragment of Bst polymerase

   Depositor

   Andrea Pauli

   Article

   Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

   Insert

   large fragment of Geobacillus stearothermophilus DNA polymerase I
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. ADORA3-Tango

   Plasmid

   #66212

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ADORA3 (ADORA3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-DIO-mCherry (AAV2)

    Viral Prep

    #50459-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-PRB

    Plasmid

    #89130

    Purpose

    mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418

    Depositor

    Elizabeth Wilson

    Article

    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

    Insert

    human progesterone receptor-B (PGR Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ-dzCas9-Act3.0

    Plasmid

    #158414

    Purpose

    CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ131A2.0

    Plasmid

    #99884

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ131A

    Plasmid

    #69273

    Purpose

    Golden Gate entry vector; 1st gRNA under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRX-IP-HaloTag7-LC3

    Plasmid

    #184899

    Purpose

    Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Tags

    HaloTag7

    Expression

    Mammalian

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Str-Ii_VSVG-SBP-EGFP

    Plasmid

    #65300

    Purpose

    synchronize trafficking of VSVG from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    VSV-G
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid Kit

    #1000000236

    Purpose

    The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.

    Depositor

    Daniel Voytas

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

18. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

    Plasmid

    #67980

    Purpose

    Cas9 activity reporter with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. SB100X in pCAG globin pA

    Plasmid

    #127909

    Purpose

    Sleeping Beauty Transposase expression vector driven by the CAG promoter

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    Sleeping Beauty Transposase
    

    Expression

    Mammalian

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRPML1-HA

    Plasmid

    #18825

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML1 (MCOLN1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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