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Showing: 661 - 680 of 172770 results

  4. lentiCas9-Blast

    Plasmid
    #52962
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Lentiviral Prep
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available Since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52962-plasmid-map-sequence-id-322376

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  8. pAAV-flex-taCasp3-TEVp (AAV5)

    Viral Prep
    #45580-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-flex-taCasp3-TEVp (#45580). In addition to the viral particles, you will also receive purified pAAV-flex-taCasp3-TEVp plasmid DNA. EF1a-driven, Cre-dependent, bicicstronic expression of designer pro-taCasp3 and TEVp. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Nirao Shah, Jim Wells
    Article
    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10
    Promoter
    EF1a
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only

  12. LX-miR

    Pooled Library
    #112200
    Purpose
    Targets 1,594 primary human miRNA genes. The library contains 4-5 gRNAs per gene and 1,000 non-targeting control gRNAs for a total of 8,382 gRNAs.
    Depositor
    Ren-Jang Lin
    Article
    Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    Lentiviral
    Available Since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. pGGG Wheat GUS marker

    Plasmid
    #165418
    Purpose
    Wheat transformation binary vector pGGG confers hygromycin resistance in wheat and contains the GUS (2x introns) reporter gene driven by OsUbi promoter
    Depositor
    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
    Article
    Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.
    Insert
    Os Ubiquitin Promoter::Gus (2x introns)::NosT
    Use
    Wheat transformation binary vector
    Promoter
    OS UbiP::GUS (2xint)::NosT
    Available Since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165418-plasmid-map-sequence-id-331269

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  14. pNeg-Ma-barnase-TAG3-TAG45

    Plasmid
    #197572
    Purpose
    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    Tags
    none
    Expression
    Bacterial
    Mutation
    3TAG and 45TAG
    Promoter
    araC and lpp
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197572-plasmid-map-sequence-id-388086

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  20. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

    Plasmid
    #216152
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216152-plasmid-map-sequence-id-437762

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Showing: 661 - 680 of 172770 results