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GST-beta-arrestin1

Plasmid

#36918

Depositor

Robert Lefkowitz

Article

Nobles et al J Biol Chem. 2007 Jul 20;282(29):21370-8

Insert

beta-arrestin1 (Arrb1 Rat)

Tags

GST

Expression

Bacterial

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only
pSLIK 3xHA-MKK6-EE neo

Plasmid

#47546

Purpose

Lentiviral expression vector for an inducible 3xHA-tagged constitutively active MKK6 mutant

Depositor

Kevin Janes

Article

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Insert

MKK6-EE (MAP2K6 Human)

Use

Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

S207E, T211E

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pBR.DT-A

Plasmid

#35955

Depositor

Jacqueline Lees

Article

Danielian et al Oncogene. 2008 Nov 20;27(51):6561-70. Ep

Insert

diphtheria toxin A

Use

Bac recombineering

Promoter

PGK

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-MCP-dNS-SRRM1-V5-mCherry

Plasmid

#235095

Purpose

SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein with MCP domain

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

pCAG-MCP-dNS-SRRM1-V5-mCherry (SRRM1 Human)

Expression

Mammalian

Mutation

The SRRM1 entry clone from DNASU was modified usi…

Promoter

CAG

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-Fos

Plasmid

#131595

Purpose

expression of mouse Fos in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Fos (Fos Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

Plasmid

#169333

Purpose

Expression of HaloTag9 at the mitochondrial surface of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

TOMM20-HaloTag9 (TOMM20 Human)

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-Cetn1/1

Plasmid

#50718

Purpose

pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Article

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Inserts

Cetn1 sgRNA1
humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh and hU6

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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TFORF0122

Plasmid

#141527

Purpose

Lentiviral vector for overexpressing the BARX1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

BARX1 (BARX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-myrlysin/BORCS5

Plasmid

#194332

Purpose

Expresses myrlysin in mammalian cells

Depositor

Juan Bonifacino

Article

Pu et al Dev Cell. 2015 Apr 20;33(2):176-88. doi:

Insert

BORCS5 (BORCS5 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-MPRA_minCMV Library

Pooled Library

#201012

Purpose

Massively parallel reporter assay (MPRA) library with minCMV promoter

Depositor

Justin English

Article

Zahm et al Nat Commun. 2024 Nov 28;15(1):10353. doi

Expression

Mammalian

Use

Luciferase

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only
AAV-GfaABC1D-lck-smV5-4x6T-WPRE

Plasmid

#196416

Purpose

Astrocytic expression of membrane-targeted V5 spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

Lck-smV5

Use

AAV; Astrocyte-selective

Tags

Lck

Expression

Mammalian

Promoter

CAG and GfaABC1D

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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3XFlagNICD4

Plasmid

#20186

Depositor

Raphael Kopan

Article

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 4 Intracellular Domain (Notch4 Mouse)

Tags

3X Flag

Expression

Mammalian

Mutation

Contains murine Notch 4 Intracellular Domain (Val…

Promoter

CMV

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only
NanoLuc-ACT1 Fusion Vector

Plasmid

#237018

Purpose

Express NanoLuc(R)-ACT1 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

ACT1 (TRAF3IP2 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET51b(+)_HaloTag9

Plasmid

#169324

Purpose

Expression of HaloTag9 in bacterial cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9

Tags

His

Expression

Bacterial

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-HA-StayGold-IRESv4-HaloTag-tdMCP-CAAX

Plasmid

#241145

Purpose

Expresses Anti-HA frankenbody-StayGold and HaloTag-tdMCP-CAAX to track mature and nascent SunTag-tagged proteins and track MS2-tagged mRNA anchored to the plasma membrane

Depositor

Tim Stasevich

Article

Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

Inserts

Anti-HA frankenbody
tdMCP - CAAX

Tags

(n2)oxStayGold(c4) E147D and HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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PM-RspA-CFAST

Plasmid

#233601

Purpose

Expression of RspA-CFAST on the PM facing the cytosol

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-CFAST

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tet2 CD

Plasmid

#72219

Purpose

Express flag-tagged mouse Tet2 catalytic domain.

Depositor

Yi Zhang

Article

Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

Insert

Ten-Eleven Translocation 2 (Tet2 Mouse)

Tags

flag

Expression

Mammalian

Mutation

The plasmid expresses TET2 catalytic domain.

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only
pET19b-EcMscL-TEV-mEGFP

Plasmid

#165097

Purpose

Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.

Depositor

Neha Kamat

Article

Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p

Insert

E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)

Use

Synthetic Biology

Tags

monomeric Enhanced Green Fluorescent Protein

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomKate2-CaURA3

Plasmid

#44878

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKate2 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKate2

Expression

Yeast

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag9_LamB1

Plasmid

#175538

Purpose

Expression of HaloTag9 at the nuclear lamina of the mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9-LamB1

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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