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168,589 results

Showing: 661 - 680 of 168589 results

  1. pET-pA-Hia5

    Plasmid
    #174372
    Purpose
    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein A - hia5
    Tags
    6xHis and Protein A
    Expression
    Bacterial
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174372-plasmid-map-sequence-id-341860

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  2. 4xNFkB Luc

    Plasmid
    #111216
    Purpose
    reporter gene
    Depositor
    Johannes A. Schmid
    Insert
    firefly Luciferase
    Expression
    Mammalian
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111216-plasmid-map-sequence-id-213082

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  4. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  9. pAAV-Syn-ChR2(H134R)-GFP (AAV Retrograde)

    Viral Prep
    #58880-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Syn-ChR2(H134R)-GFP (#58880). In addition to the viral particles, you will also receive purified pAAV-Syn-ChR2(H134R)-GFP plasmid DNA. Humanized channelrhodopsin H134R mutant fused to GFP, under the control of the Synapsin promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.
    Promoter
    Syn
    Tags
    GFP
    Available Since
    June 20, 2017
    Availability
    Academic Institutions and Nonprofits only

  10. AAV-CamKIIa-jGCaMP8m-WPRE (AAV9)

    Viral Prep
    #176751-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    CamKIIalpha
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  12. pCMV-PEmax-P2A-hMLH1dn

    Plasmid
    #174828
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Insert
    PEmax-P2A-hMLH1dn
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript; deletion of residues 754-…
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174828-plasmid-map-sequence-id-357934

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  13. pcDNA3-ELMOD2-myc-His6

    Plasmid
    #201901
    Purpose
    Used for expression of human protein ELMOD2 in mammalian cells
    Depositor
    Richard Kahn
    Insert
    ELMOD2 (ELMOD2 Human)
    Tags
    myc, 6xHis
    Expression
    Mammalian
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201901-plasmid-map-sequence-id-402532

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  15. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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  17. pAAV-ATLASsnCre (AAV8)

    Viral Prep
    #232351-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-ATLASsnCre (#232351). In addition to the viral particles, you will also receive purified pAAV-ATLASsnCre plasmid DNA. Syn-driven, ATLASsnCre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Don Arnold
    Article
    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d
    Promoter
    Syn
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only

  19. pRGEB32

    Plasmid
    #63142
    Purpose
    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.
    Depositor
    Yinong Yang
    Article
    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    Rice snoRNA U3 promoter for gRNA/PTG expression a…
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    63142-plasmid-map-sequence-id-339931

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Showing: 661 - 680 of 168589 results