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pLKO.DEST.EGFP

Plasmid

#32684

Purpose

Lentiviral cDNA/shRNA gateway destination vectors

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.DEST.EGFP

Plasmid

#32684

Purpose

Lentiviral cDNA/shRNA gateway destination vectors

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-Ataxin3Q28

Plasmid

#22125

Depositor

Henry Paulson

Insert

Ataxin 3 (ATXN3 Human)

Tags

myc

Expression

Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-myc-Ataxin3Q28

Plasmid

#22125

Depositor

Henry Paulson

Insert

Ataxin 3 (ATXN3 Human)

Tags

myc

Expression

Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
mCherry-H2A-10

Plasmid

#55054

Purpose

Localization: Nucleus/Histones, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

H2A (H2AC13 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-H2A-10

Plasmid

#55054

Purpose

Localization: Nucleus/Histones, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

H2A (H2AC13 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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Yeast Inducible CRISPRi Library

Pooled Library

#161829

Purpose

Inducible genome-wide CRISPRi library targeting S. cerevisiae genes with 6-12 guide RNAs per gene.

Depositor

Saeed Tavazoie

Article

Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

Expression

Yeast

Species

Saccharomyces cerevisiae

Use

CRISPR

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only
pQCXIP mScarlet-MOSPD2 WT

Plasmid

#186472

Purpose

Expression of human MOSPD2 fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-MOSPD2 WT

Plasmid

#186472

Purpose

Expression of human MOSPD2 fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF3083

Plasmid

#144559

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FOSL2 (FOSL2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3083

Plasmid

#144559

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FOSL2 (FOSL2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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MAC-DPP4

Plasmid

#172416

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Dipeptidyl peptidase 4 (DPP4 Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-DPP4

Plasmid

#172416

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Dipeptidyl peptidase 4 (DPP4 Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pRsetB-His7tag-Peredox-mCherry

Plasmid

#32382

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

His7-Peredox-mCherry

Tags

His7tag

Expression

Bacterial

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pRsetB-His7tag-Peredox-mCherry

Plasmid

#32382

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

His7-Peredox-mCherry

Tags

His7tag

Expression

Bacterial

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158990

Purpose

Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pCMV

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158990

Purpose

Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pCMV

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lyn11-HaloTag9

Plasmid

#175543

Purpose

Expression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lyn11-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lyn11-HaloTag9

Plasmid

#175543

Purpose

Expression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lyn11-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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V5-APEX2-SENP2

Plasmid

#129276

Purpose

Expression of APEX2 at nuclear pore

Depositor

Alice Ting

Article

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

V5-APEX2-SENP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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