169,057 results

1. pAAV-hIBA1a-GFP-miR124T

   Plasmid

   #214147

   Purpose

   AAV vector to restrict GFP expression in microglia.

   Depositor

   Chun-Li Zhang

   Article

   Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

   Has Service

   AAV5

   Insert

   GFP
   

   Use

   AAV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3/F-tractin=mStayGold

   Plasmid

   #212019

   Purpose

   For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   F-tractin
   

   Tags

   mStayGold

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. eIF2a 2

   Plasmid

   #21808

   Depositor

   David Ron

   Insert

   eIF2a (EIF2S1 Human)
   

   Expression

   Mammalian

   Mutation

   S52A

   Available Since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGLO-Blue

   Plasmid

   #220782

   Purpose

   Expresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)

   Depositor

   Angela Leu

   Insert

   blue chromoprotein (aeBlue)
   

   Tags

   8xHis

   Expression

   Bacterial

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. eIF2a 3

   Plasmid

   #21809

   Depositor

   David Ron

   Insert

   eIF2a (EIF2S1 Human)
   

   Expression

   Mammalian

   Mutation

   S52D

   Available Since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. T444T

   Plasmid

   #113081

   Purpose

   Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

   Depositor

   Tibor Vellai

   Article

   Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

   Type

   Empty backbone

   Use

   RNAi

   Expression

   Bacterial

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-HA-OTUB1

   Plasmid

   #22551

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   OTUB1 (OTUB1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. plentiCRISPRv2-sgEGFP

   Plasmid

   #86153

   Purpose

   Lentivirus carrying Cas9/CRISPR for cut in GFP (used as control)

   Depositor

   Roland Friedel

   Article

   Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

   Insert

   EGFP
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti CMV GFP Puro (658-5)

   Plasmid

   #17448

   Purpose

   3rd gen lentiviral eGFP expression vector, CMV promoter, Puro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Insert

   GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-CamKIIa-jGCaMP8m-WPRE (AAV9)

    Viral Prep

    #176751-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    CamKIIalpha

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

11. pBBR1MCS-2

    Plasmid

    #85168

    Purpose

    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

    Depositor

    Kenneth Peterson

    Article

    Kovach et al Gene. 1995 Dec 1;166(1):175-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Constitutive (derepressed P-lac)

    Available Since

    Nov. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAAV-hSyn-hChR2(H134R)-EYFP (AAV5)

    Viral Prep

    #26973-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Syn

    Tags

    EYFP

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (AAV5)

    Viral Prep

    #111568-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (#111568). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-mCherry-hPMCA2w/b plasmid DNA. GfaABCD1-driven, expression of mCherry fused plasma membrane calcium pump hPMCA2w/b. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Tags

    mCherry

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

14. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (AAV1)

    Viral Prep

    #100834-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (#100834). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 plasmid DNA. CamKII-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CaMKII

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

15. LentiCRISPRv2GFP

    Plasmid

    #82416

    Purpose

    3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning site

    Depositor

    David Feldser

    Article

    Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

    Insert

    GFP
    

    Use

    CRISPR and Lentiviral

    Promoter

    EFS (P2A)

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1 - TRC cloning vector

    Plasmid

    #10878

    Purpose

    3rd generation transfer plasmid

    Depositor

    David Root

    Article

    Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmScarlet3_C1

    Plasmid

    #189753

    Purpose

    C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. CRY2olig-mCherry

    Plasmid

    #60032

    Purpose

    Expresses a fusion of CRY2PHR E490G with mCherry

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2PHR E490G (CRY2 Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    E490G

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCXWB-EBNA1

    Plasmid

    #37624

    Purpose

    Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    EBNA-1 (EBNA-1 human herpesvirus 4)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-EF1-Luc2-P2A-tdTomato

    Plasmid

    #72486

    Purpose

    Express Luc2 and tdTomato under EF1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    Luc2-P2A-tdTomato
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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