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USP2

Plasmid

#36894

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

USP2 (USP2 Human)

Tags

His tag with thrombin cleavage

Expression

Bacterial

Available Since

June 19, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pJC49

Plasmid

#133060

Purpose

Lentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.

Depositor

Martin Kampmann

Article

Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

Insert

Tau.K18(LM)

Use

Lentiviral

Tags

mScarletI

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJC49

Plasmid

#133060

Purpose

Lentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.

Depositor

Martin Kampmann

Article

Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

Insert

Tau.K18(LM)

Use

Lentiviral

Tags

mScarletI

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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p-cyclinB1-mcherry (1958)

Plasmid

#26063

Depositor

Jonathon Pines

Article

Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

Insert

Cyclin B1 (CCNB1 Human)

Tags

mcherry

Expression

Mammalian

Available Since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

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p-cyclinB1-mcherry (1958)

Plasmid

#26063

Depositor

Jonathon Pines

Article

Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

Insert

Cyclin B1 (CCNB1 Human)

Tags

mcherry

Expression

Mammalian

Available Since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pABY-c45

Plasmid

#201771

Purpose

Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiTet-P2A-mCherry BlastR

Plasmid

#186743

Purpose

Dox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

P2A-mcherry

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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H2B-mNeonGreen-IRESpuro2

Plasmid

#183745

Purpose

Expresses H2B-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

H2B (H2BC16P Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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VPS29-YFP

Plasmid

#174520

Purpose

Expresses human VPS29 with a C-terminal YFP in mammalian cells

Depositor

Juan Bonifacino

Article

Rojas et al J Cell Biol. 2008 Nov 3;183(3):513-26. d

Insert

VPS29 (VPS29 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p-PDI

Plasmid

#202486

Purpose

Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Human, Budding Yeast)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLPT41

Plasmid

#85524

Purpose

Titration sponge for TetR

Depositor

Johan Paulsson

Article

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Type

Empty backbone

Use

Synthetic Biology

Promoter

PLtet

Available Since

April 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLPT41

Plasmid

#85524

Purpose

Titration sponge for TetR

Depositor

Johan Paulsson

Article

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Type

Empty backbone

Use

Synthetic Biology

Promoter

PLtet

Available Since

April 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3_pLyn_EGFP

Plasmid

#214605

Purpose

Expresses fluorescent protein EGFP targeted to plasma membrane

Depositor

Peter Dedecker

Article

Valenta et al Talanta. 2024 Mar

Insert

lyn-EGFP

Tags

lyn targetting

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Human, Bacillus phage PBS2, Rat)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Human, Rat, Bacillus phage PBS2)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4 [luc2P/STAT4-RE/Hygro] Vector

Plasmid

#236820

Purpose

Express STAT4 response element in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

STAT4 (STAT4 Human)

Use

Luciferase

Mutation

None

Promoter

minP

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBABE YAP1

Plasmid

#15682

Depositor

Joan Brugge

Article

Overholtzer et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pBABE YAP1

Plasmid

#15682

Depositor

Joan Brugge

Article

Overholtzer et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pHDR-FOXL2-T2A-tdTomato-PuroTK

Plasmid

#192892

Purpose

HDR donor for inserting FOXL2-T2A-tdTomato reporter

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOXL2-tdTomato (FOXL2 Human)

Use

CRISPR

Mutation

T2A-tdTomato fluorescent reporter

Promoter

PGK

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTDpelB-CTwinStrep

Plasmid

#45941

Purpose

use to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequence

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

PelB and Twin-Strep

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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