176,890 results

1. TFORF1125

   Plasmid

   #143093

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ENO1 (ENO1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. His-GIV-CTs

   Plasmid

   #69864

   Purpose

   Express His tagged GIV-CTs (1660–1870).

   Depositor

   Pradipta Ghosh

   Article

   Garcia-Marcos et al Mol Biol Cell. 2011 Mar 1;22(5):673-86.

   Insert

   GIV/Girdin (CCDC88A Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   C terminal amino acids S1660-S1870 of human GIV.

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. ERBB2 gRNA (BRDN0001148673)

   Plasmid

   #76377

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ERBB2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-RiboL1-jGCaMP8f

   Plasmid

   #192612

   Purpose

   AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8f.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   RiboL1-jGCaMP8f
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 803 pGEX2T PTEN

   Plasmid

   #10784

   Depositor

   William Sellers

   Insert

   PTEN (PTEN Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

6. 803 pGEX2T PTEN

   Plasmid

   #10784

   Depositor

   William Sellers

   Insert

   PTEN (PTEN Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pKK-TEV-BirA-FLAG

   Plasmid

   #105786

   Purpose

   Expression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-BirA-FLAG

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW-codon optimized catalytically inactive PHGDH

   Plasmid

   #154903

   Purpose

   Expresses codon optimized catalytically inactive PHGDH in mammalian cells

   Depositor

   Michael Pacold

   Article

   Ngo et al Cancer Discov. 2020 Sep;10(9):1352-1373.

   Insert

   Phosphoglycerate dehydrogenase (PHGDH Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Three mutations: D175N, R236K, H283A

   Promoter

   TRE

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRF+423Dux4

   Plasmid

   #21625

   Depositor

   Stephen Tapscott

   Article

   Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

   Insert

   Double homeobox, chr 4 (DUX4 Human)
   

   Use

   Luciferase

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pminiTol2-HuC-NE2h

    Plasmid

    #208689

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h pan-neuronally in zebrafish

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
    

    Use

    Cloning vector

    Promoter

    Huc

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFRT_TO_eGFP_ELAVL1

    Plasmid

    #106105

    Purpose

    Inducible gene expression of EGFP ELAVL1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    NP_001410.2 (ELAVL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hDlx-Flex-GFP-Fishell_6 (AAV2)

    Viral Prep

    #83895-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV-hDlx-Flex-GFP-Fishell_6 (#83895). In addition to the viral particles, you will also receive purified pAAV-hDlx-Flex-GFP-Fishell_6 plasmid DNA. hDlx-driven, Cre-dependent expression of GFP. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    hDlx

    Tags

    GFP (Cre-dependent)

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

13. pALPS puro miR30-L1221

    Plasmid

    #101335

    Purpose

    negative control for knockdowns target site: CTTGTCGATGAGAGCGTTTGT

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    shRNA targeting miR30
    

    Use

    Lentiviral

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-ATG14-C-18

    Plasmid

    #54989

    Purpose

    Localization: Membrane Receptor, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    ATG14 (ATG14 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA

    Plasmid

    #216321

    Purpose

    Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Luciferase + splice acceptor site
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Desmoplakin II-YPet(short)

    Plasmid

    #118714

    Purpose

    The human desmoplakin II is C-terminally tagged with YPet(short) to serve as wild type control.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    Desmoplakin II-YPet(short) (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid

    #208043

    Purpose

    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    SUMO2nc (SUMO2 Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Mutation

    Q90P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Gamillus

    Plasmid

    #197926

    Purpose

    Monomer expression vector

    Depositor

    Salvatore Chiantia

    Article

    Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

    Insert

    Gamillus
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pALPS puro miR30-IRF1

    Plasmid

    #117156

    Purpose

    Target site TTGCTCTTAGCATCTCGGCTG

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    shRNA targeting miR30
    

    Use

    Lentiviral

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-CreiLOV-P2A-FusionRed

    Plasmid

    #192818

    Purpose

    Expresses CreiLOV with FusionRed tag in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    CreiLOV
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Promoter

    chicken β-actin promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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