-
Plasmid#26475PurposeIn vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection markerDepositorInsertDRGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE (AAV5)
Viral Prep#130998-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE (#130998). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of ChRmine-mScarlet. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmScarletAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/2
Plasmid#104963PurposeAAV packaging plasmid expressing Rep/Cap genes for production of serotype 2DepositorInsertRep2/Cap2
UseAAVTagsExpressionMammalianMutationPromoterp5Available sinceOct. 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
mKeima-Red-Mito-7
Plasmid#56018PurposeLocalization: Mitochondria, Excitation: 440, Emission: 620DepositorInsertMito (COX8A Human)
UseTagsmKeima-RedExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE
Plasmid#60906PurposeThe plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imagingDepositorInsertscFv-GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
35S:RUBY
Plasmid#160908PurposeRUBY under the control of CaMV 35S promoter/marker for transformationDepositorInsertRUBY and CaMV 35S promoter
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJR104
Plasmid#187243PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-mCherry-IRES-Cre (AAV8)
Viral Prep#55632-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-mCherry-IRES-Cre (#55632). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry-IRES-Cre plasmid DNA. Cre and bicistronic (IRES) mCherry expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1a and Ef1a/IRESTagsmCherryAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM4D(Gi)-mCherry (AAV8)
Viral Prep#50477-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-CaMKIIa-hM4D(Gi)-mCherry (#50477). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM4D(Gi)-mCherry plasmid DNA. CaMKIIa-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-GFP-LC3-RFP-LC3ΔG
Plasmid#84572PurposeExpresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-bacteria
Plasmid#44249PurposeaTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdownDepositorInsertdCas9 (bacteria)
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterpLtetO-1Available sinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7 RNA POL
Plasmid#138352PurposeExpressing T7 RNA Polymerase in mammalian cellsDepositorInsertT7 RNA POL
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
tetO.Sox9.Puro
Plasmid#117269PurposeExpresses Sox9 and puromycin resistance gene under control of tetO sequence.DepositorInsertSox9 (Sox9 Mouse)
UseLentiviralTagsT2A and puromycin resistant cassette (C terminal …ExpressionMammalianMutationPromotertet-onAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetO-hNGN2-eGFP-Puro
Plasmid#79823PurposeExpresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.DepositorInserthNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ChrimsonR-tdT (AAV5)
Viral Prep#59171-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagstdTomatoAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003-sfGFP
Plasmid#133449PurposeExpresses SpyCatcher003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyCatcher003-sfGFP
UseTagsHis6 and TEV siteExpressionBacterialMutationPromoterT5Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV8)
Viral Prep#44362-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-28a_6H-TAQ_E602D
Plasmid#166944PurposeBacterial expression of Taq DNA polymeraseDepositorInsertThermus aquaticus DNA polymerase
UseTags6X HisExpressionBacterialMutationE602DPromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFP
Plasmid#11150PurposeMammalian expression vector for expression of GFP (CAG promoter)DepositorHas ServiceCloning Grade DNAInsertEGFP from Aequorea victoria
UseTagsExpressionMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only