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Plasmid#128395PurposeIn vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sitesDepositorInsertDiphtheria toxin
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationInactivating G52E mutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
RFP Dynamin2 Wild Type
Plasmid#128152PurposeExpression of Dynamin 2DepositorInsertDynamin 2 (Dnm2 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLostfoX-Kan
Plasmid#99865PurposeTfoX induction vector for chitin-pathway transformation (KanR)DepositorInserttfoXVF
UseV. fischeri suicide cloning vector, cmr, tcrTagsExpressionMutationPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
hCEP120-EGFP
Plasmid#50382PurposeOver-expression of EGFP-tagged hCEP120DepositorInsertHuman CEP120 (CEP120 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-PDHA1-N-10
Plasmid#58124PurposeLocalization: Mitochondria, Excitation: 554, Emission: 581DepositorInsertPDHA1 (PDHA1 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-165
Plasmid#118545PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3
Plasmid#48743PurposeExpression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1060 with respect to transcription start site at +1)DepositorInserttruncated mPer2 promoter/enhancer region (-1128 to +1060)
UseLuciferaseTagsExpressionMutationtruncated promoter region (see pGL6)PromoterAvailable sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRT-KanR-FRT pA
Plasmid#82601Purpose3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selectionDepositorInsertFRT-Kan-FRT
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLC-EGFP-RIP3
Plasmid#75165PurposeLentiCRISPR-EGFP with sgRNA targeting human RIP3DepositorInsertRIP3 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_NDRG1_ERG
Plasmid#205866PurposeExpress mEGFP-tagged fusion protein, NDRG1_ERG from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
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TFORF1168
Plasmid#143472PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertEBF2 (EBF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Ch
Plasmid#61647PurposeGateway vector for generating cyan fluorescent protein fusions in fungiDepositorInsertenhanced cyan fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
px551-mSncg-0.27kb-HA-SpCas9
Plasmid#153208PurposeTruncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cellsDepositorInsertCas9
UseAAV and CRISPRTagsHAExpressionMammalianMutationPromoterTruncated mSncg (0.27kb)Available sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF3082
Plasmid#144558PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGBX2 (GBX2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNP1 sfGFP toehold switch
Plasmid#107355PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressedDepositorInserttoehold 7 + sfGFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Rx3(A7)
Plasmid#35525DepositorInsertHis6-Rx3(A7)
UseTagsHis6ExpressionBacterialMutationChanged G2 to PPromoterT7Available sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-rNFM
Plasmid#32907PurposeMammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of rat neurofilament protein MDepositorInsertEGFP-rat NFM fusion (Nefm Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
APOH-bio-His
Plasmid#52177PurposeExpresses full-length Beta-2-glycoprotein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertAPOH (APOH Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129E
Plasmid#36050DepositorInsertalpha-synuclein S129E (SNCA Human)
UseTagsExpressionBacterialMutationS129EPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only