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Addgene
Showing: 621 - 640 of 146317 results
  1. FX cloning Kit

    Plasmid Kit
    #1000000039
    Purpose
    Insert sequence of interest into a cloning vector and subclone into expression vectors, containing a variety of N- and C-terminal tags; for use in both prokaryotic and eukaryotic systems
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  2. Platinum Gate TALEN Kit

    Plasmid Kit
    #1000000043
    Purpose
    Enables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with either +136/+63 or +153/+47 TALE scaffolds.
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  3. MoClo Tool Kit

    Plasmid Kit
    #1000000044
    Purpose
    Eukaryotic multigene construct assembly in 3 steps using modular cloning (MoClo); Has 95 empty standardized genetic modules: promoters, 5' UTRs, signal peptides, coding sequences, and terminators
    Availability
    Academic Institutions and Nonprofits only
  4. MoClo Plant Parts Kit

    Plasmid Kit
    #1000000047
    Purpose
    Modular Cloning (MoClo) collection contains 95 standardized parts to enable Golden Gate construction of multi-gene constructs for plant transformation
    Availability
    Academic Institutions and Nonprofits only
  5. MultiSite Gateway plasmid toolKit

    Plasmid Kit
    #1000000050
    Purpose
    Cloning kit for constructing fully-customizable three-part fusion proteins, based on MultiSite Gateway from Life Technologies. 12 promoter-less Gateway entry clones, N- and C-term flanking modules
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  6. PUF Assembly Kit

    Plasmid Kit
    #1000000051
    Purpose
    Used to assemble custom-made RNA-binding proteins for recognition of 8-base long targets within RNA. The kit is based on the Pumilio and FBF (PUF) homology domain of the full-length human Pumilio 1.
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  7. Golden GATEway Cloning Kit

    Plasmid Kit
    #1000000054
    Purpose
    Combinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular way
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  8. Multiplex CRISPR/Cas9 Assembly System Kit

    Plasmid Kit
    #1000000055
    Purpose
    Golden Gate cloning assembly for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase; see kit #1000000062 for FokI Accessory Pack
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  9. pCri System

    Plasmid Kit
    #1000000058
    Purpose
    pCri System: plasmids for cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli, Bacillus subtilis, and Pichia pastoris.
    Availability
    Academic Institutions and Nonprofits only
  10. CIDAR MoClo Parts Kit

    Plasmid Kit
    #1000000059
    Purpose
    CIDAR Modular Cloning (MoClo) kit uses modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design, and expression tuning in E. coli
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  11. Multiple Lentiviral Expression (MuLE) system

    Plasmid Kit
    #1000000060
    Purpose
    Multiple Lentiviral Expression (MuLE): modular system used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  12. MoClo Yeast Toolkit

    Plasmid Kit
    #1000000061
    Purpose
    96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettes
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  13. Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack

    Plasmid Kit
    #1000000062
    Purpose
    Accessory pack for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either dCas9 or dCas9-FokI fusion; Kit #1000000055 is required to use this kit
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  14. FusX TALEN assembly system

    Plasmid Kit
    #1000000063
    Purpose
    Golden Gate assembly of 15-16 RVD TALEN inserts in a single cloning step; designed to be used with high throughput robotics
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  15. MXS Chaining Kit

    Plasmid Kit
    #1000000064
    Purpose
    MXS-chaining system for assembly of constructs optimized for synthetic biology applications in mammalian systems. 14 different fluorescent proteins, 10 mammalian promoters/enhancers, 3 polyA signals
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  16. Alexandrov Cell-Free Protein Expression Kit (PEK)

    Plasmid Kit
    #1000000065
    Purpose
    Set of 89 ORFs cloned into the pCellFree vector to enable protein expression in any in vitro translation system; can perform statistically relevant benchmarking of cell-free expression systems
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  17. PRESTO-Tango Kit

    Plasmid Kit
    #1000000068
    Purpose
    Used to measure receptor activation for more than 300 G-protein coupled receptors (GPCRs) via a modified Tango beta-arrestin recruitment assay.
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  18. RAS Pathway 2.0 Clone Collection

    Plasmid Kit
    #1000000070
    Purpose
    Collection contains 180 genes in the “RAS pathway version 2.0”, a crowdsourced pathway map of the RAS signaling pathway; For use with Gateway cloning or FNLCR Combinatorial Cloning Platform
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  19. Cancer Pathways ORFs Kit

    Plasmid Kit
    #1000000072
    Purpose
    A set of vectors containing cDNAs for studying oncogenic signaling pathways. cDNAs are either wild-type, constitutively active mutants, or dominant negative mutants.
    Availability
    Academic Institutions and Nonprofits only
  20. EasyClone 2.0 Yeast Toolkit

    Plasmid Kit
    #1000000073
    Purpose
    Integrative constructs for (over-) expression of (heterologous) genes in both laboratory and industrial yeast (S. cerevisiae) strains; Allow for selection in auxotrophic and prototrophic yeast strains
    Depositor
    Availability
    Academic Institutions and Nonprofits only
Showing: 621 - 640 of 146317 results