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pDRF1-GW ymScarletI-T2A-mTurquoise2

Plasmid

#118441

Purpose

Produces equimolar expression of yeast codon-optimized mScarletI and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymScarletI-T2A-mTurquoise2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT-PonA-BI-Gl-WT-24xMS2-PTC-18xPP7 (WP)

Plasmid

#189792

Purpose

Express wild-type and PTC-containing beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

Depositor

Robert Singer

Article

Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

Insert

beta-globin (HBB Human, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ponasterone A inducible

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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HCN3/pCDNA3.1

Plasmid

#197293

Purpose

Plasmid expressing an antigen targeting the HCN3 ion channel subunit

Depositor

Dane Chetkovich

Insert

HCN3 (Hcn3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pJOE6261.2

Plasmid

#135079

Purpose

P. putida KT2440 rescue construct

Depositor

Josef Altenbuchner

Article

Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

Insert

upp (upp Pseudomonas putida)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pJB06T

Plasmid

#16008

Depositor

Fred Cross

Article

Di Talia et al Nature. 2007 Aug 23. 448(7156):947-51.

Insert

ACT1pr-DsRed.T1.N1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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TRAV16

Plasmid

#123389

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV16 (TRAV16 Human)

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV13-1

Plasmid

#123386

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV13-1 (TRAV13-1 Human)

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-WT-neo

Plasmid

#156177

Purpose

Lentiviral vector for expression of RAP2A-WT, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pNVLTv2-pvdIJD-Prha-base

Plasmid

#169244

Purpose

Suicide vector for integrating expression cassette in P. putida (L-rhamnose induction)

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

rhaRS

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhaS

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pTER_MDC1 (422-2)

Plasmid

#22191

Depositor

Eric Campeau

Insert

sh Mediator of DNA damage checkpoint 1

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pHAGE-NRAS-K135N

Plasmid

#116443

Purpose

Lentiviral expression of NRAS K135N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

K135N

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Ube2c

Plasmid

#31418

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2C Human)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron

Plasmid

#235087

Purpose

GFP splicing reporter with FRG1 intron

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron (FRG1 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

custom bidirectional

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFPc3 K-RAS 4B

Plasmid

#236452

Purpose

transient overexpression of K-RAS 4B in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

K-RAS 4B (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230

Plasmid

#225516

Purpose

Lentiviral TMEM230 expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230triplemut

Plasmid

#225521

Purpose

Lentiviral TMEM230triplemut expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mutation

Y92C/R141L/X184W

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX.(NLS)iGlucoSnFR.H66A.L276V

Plasmid

#238944

Purpose

Green glucose sensor, high sensitivity, nuclear

Depositor

Paul Khavari

Article

Lopez-Pajares et al Cell Stem Cell. 2025 Mar 20:S1934-5909(2

Insert

iGlucoSnFR.L276V.K312A

Use

Lentiviral

Tags

Myc and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX.(NLS)iGlucoSnFR.L276V.K312A

Plasmid

#238945

Purpose

Green glucose sensor, moderate sensitivity, nuclear

Depositor

Paul Khavari

Article

Lopez-Pajares et al Cell Stem Cell. 2025 Mar 20:S1934-5909(2

Insert

iGlucoSnFR.L276V.K312A

Use

Lentiviral

Tags

Myc and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLT3-D835Y-V5/HIS

Plasmid

#236007

Purpose

expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

D835Y substitution

Promoter

CMV

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLT3-D835N-V5/HIS

Plasmid

#236008

Purpose

expression of the D835N kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FLT3-D835N receptor tyrosine kinase, full length (FLT3 Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

D835N substitution

Promoter

CMV

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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