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pYPQ132-STU-As

Plasmid

#138097

Purpose

Golden Gate entry vector for 2nd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ133-STU-As

Plasmid

#138100

Purpose

Golden Gate entry vector for 3rd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pBW_0007

Plasmid

#102839

Purpose

Binary vector containing Sr22 PI573523 allele driven by Sr33 promoter, Sr33 terminator

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

Sr22 PI573523 (NEWENTRY Synthetic)

Use

Tags

Expression

Plant

Mutation

Promoter

Sr33 promoter

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBW_0002

Plasmid

#102834

Purpose

Binary vector containing Sr22 Schomburgk allele driven by Sr33 promoter, Sr33 terminator

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

Sr22 Schomburgk (NEWENTRY Synthetic)

Use

Tags

Expression

Plant

Mutation

Promoter

Sr33 promoter

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBW_0003

Plasmid

#102835

Purpose

Binary vector containing Sr22 Schomburgk allele driven by Sr33 promoter, Sr22 terminator

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

Sr22 Schomburgk (NEWENTRY Synthetic)

Use

Tags

Expression

Plant

Mutation

Promoter

Sr33 promoter

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBW_0004

Plasmid

#102836

Purpose

Binary vector containing Sr22 PI190945 allele driven by Sr33 promoter, Sr33 terminator

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

Sr22 PI190945 (NEWENTRY Synthetic)

Use

Tags

Expression

Plant

Mutation

Promoter

Sr33 promoter

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW_0005

Plasmid

#102837

Purpose

Binary vector containing Sr22 PI573523 allele driven by maize ubiquitin promoter, Sr22 terminator

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

Sr22 PI573523 (NEWENTRY Synthetic)

Use

Tags

Expression

Plant

Mutation

Promoter

Maize ubiquitin

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBW_0006

Plasmid

#102838

Purpose

Binary vector containing Sr22 Schomburgk allele driven by Sr33 promoter, Sr33 promoter (undomesticated)

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

Sr22 Schomburgk (NEWENTRY Synthetic)

Use

Tags

Expression

Plant

Mutation

Promoter

Sr33 promoter

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW_0141

Plasmid

#102820

Purpose

pVec8 containing Sr45 gene

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

wheat stem rust resistance gene Sr45 (NEWENTRY Synthetic)

Use

Tags

Expression

Plant

Mutation

Promoter

Sr33

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pPK-352

Plasmid

#157922

Purpose

pcDNA-CMV-PCYA-IRES-HO1-P2A-FD-P2A-FNR

Depositor

Phillip Kyriakakis

Article

Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

Inserts

tPcyA
IRES
HO1
FD
FNR

Use

Synthetic Biology; Optogenetics

Tags

Mitochondrial Targeting Sequence, Mitochondrial T…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pDONRG_P4-P1R:TBA2p

Plasmid

#128429

Purpose

Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in Three-way Gateway cloning.

Depositor

George Haughn

Article

McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

Insert

TESTA ABUNDANT 2 (AT1G62060 Mustard Weed)

Use

Gateway promoter entry clone

Tags

Expression

Mutation

Promoter

TESTA ABUNDANT 2

Available since

Oct. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR CMVie-Lifeact-EGFP L1-R5

Plasmid

#84391

Purpose

Multisite Gateway entry vector for expression of EGFP-tagged Lifeact in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Gateway

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-TER+-shDIXDC1-Hu-16

Plasmid

#61237

Purpose

Entry clone encoding shRNA to human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

Expression

Mutation

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-MYC-hDIXDC1-L-F630S

Plasmid

#61241

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

MYC

Expression

Mutation

Changed Phenylalanine 630 to Serine

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-MYC-hDIXDC1-L-S592A

Plasmid

#61240

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

MYC

Expression

Mutation

Changed Serine 592 to Alanine

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-MYC-hDIXDC1-L-WT

Plasmid

#61239

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

MYC

Expression

Mutation

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-FLAG-hDIXDC1-L-S592A

Plasmid

#61211

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

FLAG

Expression

Mutation

Changed Serine 592 to Alanine

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-FLAG-hDIXDC1-L-F630S

Plasmid

#61212

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

FLAG

Expression

Mutation

Changed Phenylalanine 630 to Serine

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-FLAG-hDIXDC1-L-WT

Plasmid

#61210

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

FLAG

Expression

Mutation

Promoter

None

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pGEX4T-1 hSlp4-a W118S

Plasmid

#40065

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a W118S (SYTL4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Tryptophane 118 to Serine

Promoter

Tac

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only