gp64-mVEGF-C-HisTag-pENTR
(Plasmid
#64065)
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PurposePlasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64065 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR/D-TOPO
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Backbone manufacturerInvitrogen
- Total vector size (bp) 3027
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namevascular endothelial growth factor C
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Alt nameVEGF-C
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SpeciesM. musculus (mouse)
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Insert Size (bp)440
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GenBank IDU73620.1
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Entrez GeneVegfc (a.k.a. AW228853, VEGF-, VEGF-C)
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Tag
/ Fusion Protein
- His-tag (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDNA encoding a mature form of murine VEGF-C was amplified by PCR from a plasmid, which was a kind gift from Prof. Melody A. Swartz (Ecole Polytechnique Fédérale de Lausanne).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
gp64-mVEGF-C-HisTag-pENTR was a gift from Joanna Bereta (Addgene plasmid # 64065 ; http://n2t.net/addgene:64065 ; RRID:Addgene_64065) -
For your References section:
Overcoming inefficient secretion of recombinant VEGF-C in baculovirus expression vector system by simple purification of the protein from cell lysate. Klaus T, Kulesza M, Bzowska M, Wyroba B, Kilarski WW, Bereta J. Protein Expr Purif. 2015 Mar 7. pii: S1046-5928(15)00040-6. doi: 10.1016/j.pep.2015.03.001. 10.1016/j.pep.2015.03.001 PubMed 25758709