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Addgene

gp64-mVEGF-C-HisTag-pENTR
(Plasmid #64065)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64065 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pENTR/D-TOPO
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 3027
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    vascular endothelial growth factor C
  • Alt name
    VEGF-C
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    440
  • GenBank ID
    U73620.1
  • Entrez Gene
    Vegfc (a.k.a. AW228853, VEGF-, VEGF-C)
  • Tag / Fusion Protein
    • His-tag (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DNA encoding a mature form of murine VEGF-C was amplified by PCR from a plasmid, which was a kind gift from Prof. Melody A. Swartz (Ecole Polytechnique Fédérale de Lausanne).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    gp64-mVEGF-C-HisTag-pENTR was a gift from Joanna Bereta (Addgene plasmid # 64065 ; http://n2t.net/addgene:64065 ; RRID:Addgene_64065)
  • For your References section:

    Overcoming inefficient secretion of recombinant VEGF-C in baculovirus expression vector system by simple purification of the protein from cell lysate. Klaus T, Kulesza M, Bzowska M, Wyroba B, Kilarski WW, Bereta J. Protein Expr Purif. 2015 Mar 7. pii: S1046-5928(15)00040-6. doi: 10.1016/j.pep.2015.03.001. 10.1016/j.pep.2015.03.001 PubMed 25758709