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Plasmid#205236PurposeExpresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85betaDepositorUseTagsNuclear Export Sequence and mCherryExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only
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pTarget TMEM97-P2A-mCardinal
Plasmid#157747PurposeExpress in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.DepositorInsertTMEM97 (TMEM97 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
DMP1-GFPtopaz
Plasmid#114270PurposeGFP labeling of mature osteoblasts and osteocytes.DepositorInsertDmp1 + GFPtopaz (Dmp1 Mouse, Aequorea victoria)
UseTagsExpressionMammalianMutationPromoterDmp1Available sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn ChR E90R-D156N-T159C 2A tDimer
Plasmid#52494PurposeHistorical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)DepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVTagsExpressionMammalianMutationE90R, D156N, T159CPromoterhuman synapsin-1 and ribosomal skip sequenceAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Trib3
Plasmid#131157PurposeMammalian expression of flag-tagged mouse TRIB3DepositorInsertTrib3 (Trib3 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jip1 JBD
Plasmid#15751Purposebinds to JnkDepositorInsertJip1 (Jnk binding domain) (Mapk8ip1 Mouse)
UseTagsFlagExpressionMammalianMutationdeleted 1-126 and 282-660PromoterAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn ChR E90R-T159C 2A tDimer
Plasmid#52495PurposeHistorical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)DepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVTagsExpressionMammalianMutationE90R, T159CPromoterhuman synapsin-1 and ribosomal skip sequenceAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
LacI/109-pHG165c
Plasmid#90058PurposeWT LacI polymorphism with T at position 109DepositorInsertLacI/109
UseTagsExpressionBacterialMutation109PromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
DEL-pHG165a
Plasmid#90064PurposeLLhG coding region on pHG165a interrupted with a single base-pair deletion, 'no repressor' control plasmidDepositorInsertDEL
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
RRRAA-5-mut-pHG165c
Plasmid#170113PurposeConstitutively expressed, mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.DepositorInsertRRRAA-5-MUT
UseTagsExpressionBacterialMutation5A14L, W116Q, E178K, S184T, S237LPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gal4-HSF4b WT
Plasmid#118358PurposeThis plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.DepositorInserthHSF4b (HSF4 Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
LLhT-pHG165a V52A
Plasmid#90050PurposeConstitutive expression of a chimeric LacI:TreR transcription repressorDepositorInsertLLhT/V52A
UseTagsExpressionBacterialMutationV52APromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhA-pHG165a Q55A
Plasmid#90052PurposeConstitutive expression of a chimeric LacI:AscG transcription repressorDepositorInsertLLhA/Q55A
UseTagsExpressionBacterialMutationQ55APromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhC-pHG165a Q55I
Plasmid#90053PurposeConstitutive expression of a chimeric LacI:CytR transcription repressorDepositorInsertLLhC/Q55I
UseTagsExpressionBacterialMutationQ55IPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LacI-11/109-pHG165c
Plasmid#90059PurposeDimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109DepositorInsertLacI-11/109
UseTagsExpressionBacterialMutation109PromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(C631S, C643S and C699T)
Plasmid#52128Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(M1-5) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationC631S, C643S and C699TPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 T7 Jip1b(471-707)
Plasmid#52428Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(471-707) ) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-470PromoterAvailable sinceApril 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(282-707)
Plasmid#52126Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(282-707)) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-281PromoterAvailable sinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip1(1-470)
Plasmid#51724Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 471-660PromoterAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip1(471-660)
Plasmid#51766Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 1-470PromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only