pcDNA-Flag-Trib3
(Plasmid #131157)

• Purpose: Mammalian expression of flag-tagged mouse TRIB3
• Depositing Labs: Karen Keeshan, Warren Pear
• Publication: Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 10.1182/blood-2009-07-229450. Epub 2010 Apr 21.

Backbone

• Vector backbone: pcDNA3
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Trib3
• Species: M. musculus (mouse)
• Entrez Gene: Trib3 (a.k.a. Ifld2, Nipk, SINK, SKIP3, TRB-3, Trb3)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Murine Trib3 with an N-terminal Flag tag cloned into BamHI-EcoRI site (with Bgl2 so BamHI site is destroyed). Release insert with HindIII and XhoI.