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PurposeMammalian expression of rat LC3 fused to mRFP and EGFP
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 21074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1+mRFP
- Backbone size w/o insert (bp) 5200
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemicrotubule-associated protein 1 light chain 3 beta
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Alt nameMap1lc3b
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Alt nameLC3
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Alt nameLC3b
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SpeciesR. norvegicus (rat)
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Insert Size (bp)738
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GenBank IDU05784
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Entrez GeneMap1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
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Tags
/ Fusion Proteins
- mRFP (N terminal on backbone)
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Compbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharian DA, Tsien RY, A monomeric red fluorescent protein, Proc Natl Acad Sci USA. 2002, 99(12):7877-82
NOTE FROM AUTHORS: We found several alterations between our plasmid and the version on NCBI [Map1lc3b; https://www.ncbi.nlm.nih.gov/gene/64862) ].
Our original clone may be a kind of variant.
In the C-terminal end of tfLC3, the stop codon is removed, which causes an additional 17 amino acid residues.
However, the C-terminal end from Gly120, including the 17 amino acids, is immediately cut off by Atg4 protease after the translation.
Therefore that sequence variant will not affect the function of the protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ptfLC3 was a gift from Tamotsu Yoshimori (Addgene plasmid # 21074 ; http://n2t.net/addgene:21074 ; RRID:Addgene_21074) -
For your References section:
Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3. Kimura S, Noda T, Yoshimori T. Autophagy. 2007;3(5):452-60. 10.4161/auto.4451 PubMed 17534139