LacI-11/109-pHG165c
(Plasmid #90059)

• Purpose: Dimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109
• Depositing Lab: Liskin Swint-Kruse
• Publication: Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139-54. doi: 10.1093/nar/gks806. Epub 2012 Sep 10.

Backbone

• Vector backbone: phG165c
• Backbone manufacturer: PMID: 3012611 with residual LacI C-terminal codons of pHG165 removed
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: LacI-11/109
• Species: E. coli
• Mutation: 109
• Promoter: Iq

Cloning Information

• Cloning method: Ligation Independent Cloning

Resource Information

• Supplemental Documents:
  • Annotated sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

WT LacI variant, stop codon inserted 11 amino acids from 3'-end of the gene; polymorphism at position 109 with T . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.