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pDONR223-PRKCQ

Plasmid

#23424

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKCQ (PRKCQ Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-SCYL2

Plasmid

#23458

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SCYL2 (SCYL2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLVX-KIF2C

Plasmid

#205996

Purpose

inducible expression of HA-KIF2C

Depositor

Floris Foijer

Article

Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

Insert

KIF2c (KIF2C Human)

Tags

HA

Expression

Mammalian

Promoter

pTight

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-YES1

Plasmid

#23938

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

YES1 (YES1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PKMYT1

Plasmid

#23884

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PKMYT1 (PKMYT1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PKM2

Plasmid

#23757

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PKM2 (PKM Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
CS-TRE-c-Myc-Ubc-rtTA

Plasmid

#198190

Purpose

Generation of Expandable megakaryocyte cell lines from human iPS cells

Depositor

Hiromitsu Nakauchi

Article

Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

Insert

hC-MYC (MYC Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)

Plasmid

#145843

Purpose

Lentiviral vector genome to ectopically express human TMPRSS2 variant 1

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human TMPRSS2 variant 1 (TMPRSS2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G8V

Promoter

SFFV

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-KIF2C

Plasmid

#205994

Purpose

Over-expression of GFP-KIF2c

Depositor

Floris Foijer

Article

Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

Insert

KIF2c (KIF2C Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa2 WT

Plasmid

#69826

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAF1_WT

Plasmid

#82317

Purpose

Gateway Donor vector containing RAF1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAF1 (RAF1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET30-2-GPD1

Plasmid

#83911

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-STK24

Plasmid

#23595

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK24 (STK24 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pAB1676 REPAIR.t1

Plasmid

#176319

Purpose

CMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Inserts

human codon optimized Cas13bt1 (catalytically inactivated)
huADAR2dd(E488Q) (ADARB1 Human)

Use

CRISPR

Tags

HIV NES

Expression

Mammalian

Mutation

E488Q, only the deaminase domain (aa 276-702 are …

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO_HA.AU1.V5_NGFR

Plasmid

#158235

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

HA.AU1.V5

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKAG3

Plasmid

#23549

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAG3 (PRKAG3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PDK4

Plasmid

#23577

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDK4 (PDK4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKCA

Plasmid

#23550

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKCA (PRKCA Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-STK38

Plasmid

#23811

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK38 (STK38 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-kappa-myc-dL5-2xG4S-TMst

Plasmid

#73206

Purpose

Expresses myc-dL5(E52D)-TM (PDGFR derived) on the surface of mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)

Depositor

Marcel Bruchez

Article

Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

Insert

kappa-myc-dL5-2XG4S-TM (MYC Human)

Tags

The FAP is fused with 2 copies of a G4S linker an…

Expression

Mammalian

Mutation

The dL5** FAP is E50D, L89S, also known as E52D/L…

Promoter

CMV

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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