We narrowed to 11,784 results for: 110

1. pLKO_S.V5.HA_NGFR

   Plasmid

   #158339

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   S.V5.HA

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-NEK11

   Plasmid

   #23816

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   NEK11 (NEK11 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pDONR223-SNRK

   Plasmid

   #23405

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   SNRK (SNRK Human)
   

   Use

   Gateway donor vector

   Mutation

   A290G,C346G

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pLKO_Ollas.AU1.C_NGFR

   Plasmid

   #158314

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Ollas.AU1.C

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_MYC_p.E366Q

   Plasmid

   #82819

   Purpose

   Gateway Donor vector containing MYC, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   MYC (MYC Human)
   

   Use

   Gateway entry vector

   Mutation

   L1M; E366Q

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSumo24P7vB4

   Plasmid

   #113073

   Purpose

   Plasmid for highly efficient expression of engineered IL24 with binding affinity to cognate receptors

   Depositor

   Bohdan Schneider

   Article

   Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:

   Insert

   Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 Human, SUMO part (Brachypodium distachyon))
   

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…

   Promoter

   T7

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO_HA.AU1.V5_NGFR

   Plasmid

   #158235

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   HA.AU1.V5

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223-PIP5KL1

   Plasmid

   #23631

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PIP5KL1 (PIP5KL1 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pDONR223-PXK

   Plasmid

   #23723

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PXK (PXK Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pDONR221 - myc ULK1 k46I

    Plasmid

    #27624

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 K46I (Myc Mouse)
    

    Use

    Gateway donr vector

    Tags

    myc

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Available Since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pDONR223-PMVK

    Plasmid

    #23771

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PMVK (PMVK Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pSumo24P7

    Plasmid

    #113072

    Purpose

    Plasmid for highly efficient expression of engineered IL24 with mutated binding sites

    Depositor

    Bohdan Schneider

    Article

    Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:

    Insert

    Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 Human, SUMO part (Brachypodium distachyon))
    

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…

    Promoter

    T7

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR223-STK17B

    Plasmid

    #23721

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    STK17B (STK17B Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pDONR223-ULK2

    Plasmid

    #23474

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ULK2 (ULK2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

15. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid

    #136659

    Purpose

    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223-FLJ23356

    Plasmid

    #23375

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    FLJ23356 (POMK Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pDONR223-PI4KAP2

    Plasmid

    #23601

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PI4KAP2 (PI4KAP2 Human)
    

    Use

    Gateway donor vector

    Mutation

    V154G

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

18. Anti-Ataxin-1, 11NQ [N76/3R]

    Plasmid

    #114559

    Purpose

    Mammalian Expression Plasmid of anti-Ataxin-1, 11NQ (Mouse). Derived from hybridoma N76/3.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Ataxin-1, 11NQ (Mus musculus) recombinant mouse monoclonal antibody (Atxn1 Mouse)
    

    Expression

    Mammalian

    Promoter

    dual CMV

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. CRY2low-mCherry-Raf1

    Plasmid

    #104066

    Purpose

    Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry and Raf1

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2low-mCherry-Raf1 (CRY2, RAF1 Human, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    CRY2 aa 1-491 truncation, R489E A491D

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-FLT4-F131S

    Plasmid

    #116401

    Purpose

    Lentiviral expression of FLT4 F131S

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FLT4 (FLT4 Human)
    

    Use

    Lentiviral

    Mutation

    F131S

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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