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C-term png in pGEX4

Plasmid

#112975

Purpose

For protein expression and purification of the C-term of Drosophila png

Depositor

Terry Orr-Weaver

Publication

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png (png Fly)

Expression

Bacterial

Mutation

wild-type C terminus

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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C2 png genomic in Casper

Plasmid

#112971

Purpose

P element vector for transposon insertion of png genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Publication

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png genomic DNA (png Fly)

Use

P insertion vector

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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C2 png genomic in Casper

Plasmid

#112971

Purpose

P element vector for transposon insertion of png genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Publication

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png genomic DNA (png Fly)

Use

P insertion vector

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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C2 M2 png-myc in Casper4

Plasmid

#112968

Purpose

P element vector for transposon insertion of Myc-tagged png into Drosophila genome

Depositor

Terry Orr-Weaver

Publication

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png-myc (png Fly)

Use

P insertion vector

Tags

myc epitope

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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C2 M2 png-myc in Casper4

Plasmid

#112968

Purpose

P element vector for transposon insertion of Myc-tagged png into Drosophila genome

Depositor

Terry Orr-Weaver

Publication

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png-myc (png Fly)

Use

P insertion vector

Tags

myc epitope

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103 WWP1 WW domain #4

Plasmid

#104223

Purpose

Bacterial expression of WW domain #4 from WWP1

Depositor

Sachdev Sidhu, Marius Sudol

Insert

WWP1 WW domain #4 (WWP1 Human)

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103 WWP1 WW domain #4

Plasmid

#104223

Purpose

Bacterial expression of WW domain #4 from WWP1

Depositor

Sachdev Sidhu, Marius Sudol

Insert

WWP1 WW domain #4 (WWP1 Human)

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103 WWP1 WW domain #3

Plasmid

#104222

Purpose

Bacterial expression of WW domain #3 from WWP1

Depositor

Sachdev Sidhu, Marius Sudol

Insert

WWP1 WW domain #3 (WWP1 Human)

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103 WWP1 WW domain #3

Plasmid

#104222

Purpose

Bacterial expression of WW domain #3 from WWP1

Depositor

Sachdev Sidhu, Marius Sudol

Insert

WWP1 WW domain #3 (WWP1 Human)

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103 WWP1 WW domain #1

Plasmid

#104220

Purpose

Bacterial expression of WW domain #1 from WWP1

Depositor

Sachdev Sidhu, Marius Sudol

Insert

WWP1 WW domain #1 (WWP1 Human)

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103 WWP1 WW domain #1

Plasmid

#104220

Purpose

Bacterial expression of WW domain #1 from WWP1

Depositor

Sachdev Sidhu, Marius Sudol

Insert

WWP1 WW domain #1 (WWP1 Human)

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103 WWP1 WW domain #2

Plasmid

#104221

Purpose

Bacterial expression of WW domain #2 from WWP1

Depositor

Sachdev Sidhu, Marius Sudol

Insert

WWP1 WW domain #2 (WWP1 Human)

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103 WWP1 WW domain #2

Plasmid

#104221

Purpose

Bacterial expression of WW domain #2 from WWP1

Depositor

Sachdev Sidhu, Marius Sudol

Insert

WWP1 WW domain #2 (WWP1 Human)

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
10xHis-ataxin-3 *SIM-HA

Plasmid

#89983

Purpose

Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Publication

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

10xHis and HA

Expression

Mammalian

Mutation

mutated aa 162IFVV to 162AFAA

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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10xHis-ataxin-3 *SIM-HA

Plasmid

#89983

Purpose

Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Publication

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

10xHis and HA

Expression

Mammalian

Mutation

mutated aa 162IFVV to 162AFAA

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-ataxin-3 *SIM

Plasmid

#89979

Purpose

Expresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Publication

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutated 162IFVV to 162AFAA

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-ataxin-3 *SIM

Plasmid

#89979

Purpose

Expresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Publication

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutated 162IFVV to 162AFAA

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_ETFB_WT_V5

Plasmid

#82987

Purpose

Gateway Donor vector containing ETFB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ETFB (ETFB Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ETFB_WT_V5

Plasmid

#82987

Purpose

Gateway Donor vector containing ETFB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ETFB (ETFB Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_CHD2_p.P474R

Plasmid

#81474

Purpose

Gateway Donor vector containing CHD2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CHD2 (CHD2 Human)

Use

Gateway Cloning

Mutation

P474R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge