pDONR223-AXL
(Plasmid #23945)

• Depositing Labs: William Hahn, David Root
• Publication: Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627

Backbone

• Vector backbone: pDONR223
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5005
• Vector type: Gateway Donor vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AXL
• Alt name: AXL receptor tyrosine kinase
• Species: H. sapiens (human)
• Insert Size (bp): 2658
• Mutation: Q31P, Q764R
• Entrez Gene: AXL (a.k.a. ARK, AXL3, JTK11, Tyro7, UFO)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13pUC-fwd
• 3′ sequencing primer: M13rev

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The ORF has a Stop codon.

A set of 4 custom lentiviral vectors is available (pLX301 http://www.addgene.org/25895 , pLX302 http://www.addgene.org/25896 , pLX303 http://www.addgene.org/25897 , pLX304 http://www.addgene.org/25890 ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

Addgene QC NGS analysis identifies a Q31P and Q764R mutation relative to NP_068713.2. As the Kinase ORFeome collection has been generated by high throughput methods using RNA from different tissues and sources, it is possible that this is a tissue specific isoform.