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Plasmid#170822PurposePiggyBac Cas13d sgRNA plasmid for PPP2CA knockdownDepositorInsertCas13d PPP2CA gRNA1
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-Tet-on 3G-Puro U6 BbsI
Plasmid#159756PurposePiggybacTransposon-based expression control of Tet-on 3G, Puro and Cas13d U6 gRNA backboneDepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac-TetO-En1-t2a-Foxa2-hygro
Plasmid#176483PurposeTetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistanceDepositorUseTagsExpressionMammalianMutationPromoterTetOAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac-TetO-Lmx1a-t2a-Pitx3-puro
Plasmid#176484PurposeTetO-Lmx1a-t2a-Pitx3 in a PiggyBac vector with puromycin resistanceDepositorUseTagsExpressionMammalianMutationPromoterTetOAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)
Plasmid#209084PurposeNgn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expressionDepositorInsertsUseTagsExpressionMammalianMutationhuman codon optimizedPromoterhSynAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)
Plasmid#209085PurposeNgn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expressionDepositorUseTagsExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggybac-Syn-mScn8a_ICL1-sfGFP-P2A-mCherry
Plasmid#182560PurposeExpresses Nav1.6 ICL1 tagged with sfGFP-P2A-mCherryDepositorInsertsmouse Scn8a ICL1
sfGFP-P2A-mCherry
UseTagsExpressionMammalianMutationPromoterSynAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)
Plasmid#209086PurposeNgn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expressionDepositorUseTagsAviTagExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)
Plasmid#209079PurposeNgn2 mediated cortical neuron induction, along with sfGFP over-expressionDepositorInsertsUseTagsExpressionMammalianMutationPromoterTRE4GAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)
Plasmid#209077PurposeNgn2 mediated cortical neuron induction, dox induction is includedDepositorInsertsUseTagsExpressionMammalianMutationPromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4
Plasmid#217971PurposeProvides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.DepositorInsert2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)
Plasmid#209082PurposeNFIB and SOX9 mediated astrocye inductionDepositorUseTagsExpressionMammalianMutationPromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)
Plasmid#209078PurposeNgn2 mediated cortical neuron induction, along with SNCA(3K) over-expressionDepositorUseTagssfGFPExpressionMammalianMutation3K mutant: E35K, E46K, E61KPromoterTRE4GAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)
Plasmid#209080PurposeNgn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expressionDepositorUseTagssfGFPExpressionMammalianMutationA53TPromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)
Plasmid#209081PurposeNgn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expressionDepositorUseTagssfGFPExpressionMammalianMutationA53T, NAC deletion (aa60-95)PromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-PB
Plasmid#133885PurposepiggyBac ITR's in high copy number pBluescript backbone plasmidDepositorTypeEmpty backboneUsePiggybacTagsExpressionMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
p15A-PB
Plasmid#133886PurposepiggyBac ITR's in low copy number p15A backbone plasmidDepositorTypeEmpty backboneUsePiggybacTagsExpressionMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-minimal PB
Plasmid#177151PurposeMinimal piggyBac ITR's in high copy number pBluescript backbone plasmidDepositorTypeEmpty backboneUsePiggybacTagsExpressionMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-MCS-Puro
Plasmid#219794PurposeMammalian expression plasmids using piggyBac Transposon SystemDepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-CA retrofitted
Plasmid#159093PurposepiggyBac ITR's from PB-CA retrofitted with a linkerDepositorTypeEmpty backboneUsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only