piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)
(Plasmid
#209080)
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PurposeNgn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209080 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepiggyBac-rtTA (4th_Gen)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameSNCA
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SpeciesH. sapiens (human)
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MutationA53T
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Entrez GeneSNCA (a.k.a. NACP, PARK1, PARK4, PD1)
- Promoter TRE4G
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Tag
/ Fusion Protein
- sfGFP (C terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Gateway Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameNGN2
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SpeciesM. musculus (mouse)
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Entrez GeneNeurog2 (a.k.a. Atoh4, Math4A, bHLHa8, ngn-2, ngn2)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
VK_1123
Please visit https://doi.org/10.1101/2022.11.08.515615 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123) was a gift from Vikram Khurana (Addgene plasmid # 209080 ; http://n2t.net/addgene:209080 ; RRID:Addgene_209080) -
For your References section:
Rapid iPSC inclusionopathy models shed light on formation, consequence and molecular subtype of alpha-synuclein inclusions. Lam et al.. bioRxiv 2022 10.1101/2022.11.08.515615