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179,538 results

Showing: 41 - 60 of 179538 results

  1. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid
    #62987
    Purpose
    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)
    Depositor
    Feng Zhang
    Publication
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9n-2A-Puro
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Cas9 D10A nickase mutant
    Available since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    119 Articles
    62987-plasmid-map-sequence-id-435258

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  2. pCW57.1

    Plasmid
    #41393
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector, doxycycli…
    Expression
    Mammalian
    Promoter
    TRE promoter, Tet ON
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    248 Articles
    41393-plasmid-map-sequence-id-364440

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  4. pAAV2/9n

    Plasmid
    #112865
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap9
    Use
    AAV
    Promoter
    Truncated P5
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115 Articles
    112865-plasmid-map-sequence-id-350300

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  5. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV Retrograde)

    Viral prep
    #105553-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  6. pcDNA3 APEX2-NES

    Plasmid
    #49386
    Purpose
    Expresses APEX2 in the cytosol of mammalian cells
    Depositor
    Alice Ting
    Publication
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Flag - APEX2 - NES
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K14D, W41F, E112K, A134P on soybean APX; improved…
    Promoter
    CMV
    Available since
    Nov. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74 Articles
    49386-plasmid-map-sequence-id-327822

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  7. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV1)

    Viral prep
    #105553-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only

  10. SP-dCas9-VPR

    Plasmid
    #63798
    Purpose
    SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector
    Depositor
    George Church
    Publication
    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SP-dCas9-VPR
    Use
    CRISPR and Synthetic Biology
    Tags
    VPR (VP64-p65-Rta)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124 Articles
    63798-plasmid-map-sequence-id-258220

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  11. pAAV.TBG.PI.eGFP.WPRE.bGH (AAV8)

    Viral prep
    #105535-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV.TBG.PI.eGFP.WPRE.bGH (#105535). In addition to the viral particles, you will also receive purified pAAV.TBG.PI.eGFP.WPRE.bGH plasmid DNA. TBG-driven eGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    TBG
    Tags
    EGFP
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. pHR-SFFV-dCas9-BFP-KRAB

    Plasmid
    #46911
    Purpose
    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain
    Depositor
    Stanley Qi, Jonathan Weissman
    Publication
    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008
    Insert
    dCas9-BFP-KRAB fusion
    Use
    CRISPR and Lentiviral
    Tags
    2xNLS, BFP, HA, and KRAB domain
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120 Articles
    46911-plasmid-map-sequence-id-449615

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  14. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV5)

    Viral prep
    #44361-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Publication
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pLVTHM

    Plasmid
    #12247
    Purpose
    2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid
    Depositor
    Didier Trono
    Publication
    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    194 Articles
    12247-plasmid-map-sequence-id-352425

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  19. ptfLC3

    Plasmid
    #21074
    Purpose
    Mammalian expression of rat LC3 fused to mRFP and EGFP
    Depositor
    Tamotsu Yoshimori
    Publication
    Kimura et al Autophagy. 2007;3(5):452-60.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    Tags
    EGFP and mRFP
    Expression
    Mammalian
    Available since
    May 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    330 Articles
    21074-plasmid-map-sequence-id-324322

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Showing: 41 - 60 of 179538 results