266,399 results
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Plasmid#48140PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorInserthSpCas9n
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-dR8.2 dvpr
Plasmid#8455Purpose2nd* generation lentiviral packaging plasmid. (*See comments section.) Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.DepositorPublicationHas serviceCloning Grade DNATypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-dR8.2 dvpr
Plasmid#8455Purpose2nd* generation lentiviral packaging plasmid. (*See comments section.) Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.DepositorPublicationHas serviceCloning Grade DNATypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)
Plasmid#42335PurposeA human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.DepositorPublicationHas serviceCloning Grade DNAInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)
Plasmid#42335PurposeA human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.DepositorPublicationHas serviceCloning Grade DNAInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9(BB)-2A-Puro (PX459)
Plasmid#48139PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.DepositorInserthSpCas9-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9(BB)-2A-Puro (PX459)
Plasmid#48139PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.DepositorInserthSpCas9-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-pegRNA-GG-acceptor
Plasmid#132777PurposePrime editing in mammalian cellsDepositorInsertExchangeable cassette
ExpressionMammalianMutationSee manuscriptPromoterU6Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-pegRNA-GG-acceptor
Plasmid#132777PurposePrime editing in mammalian cellsDepositorInsertExchangeable cassette
ExpressionMammalianMutationSee manuscriptPromoterU6Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO.1 puro
Plasmid#8453Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.DepositorPublicationHas serviceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiExpressionMammalianAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO.1 puro
Plasmid#8453Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.DepositorPublicationHas serviceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiExpressionMammalianAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
M51 Super 8x FOPFlash (TOPFlash mutant)
Plasmid#12457PurposeControl for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.DepositorInsertmutant TCF/LEF binding sites (Ctnnb1 )
UseLuciferaseExpressionMammalianMutationmutant TCF/LEF binding sitesAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
M51 Super 8x FOPFlash (TOPFlash mutant)
Plasmid#12457PurposeControl for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.DepositorInsertmutant TCF/LEF binding sites (Ctnnb1 )
UseLuciferaseExpressionMammalianMutationmutant TCF/LEF binding sitesAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK793
Plasmid#8827DepositorInsertTEV protease, S219V mutant
TagsHis, MBP (with TEV), and polyarginineExpressionBacterialMutationS219V mutation (improves stability)Available sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK793
Plasmid#8827DepositorInsertTEV protease, S219V mutant
TagsHis, MBP (with TEV), and polyarginineExpressionBacterialMutationS219V mutation (improves stability)Available sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA_Cloning Vector
Plasmid#41824PurposeAn empty gRNA expression vector, used to create a gRNA to a specific sequence.DepositorHas serviceCloning Grade DNATypeEmpty backboneUseCRISPRTagsAflII site for Gibson AssemblyExpressionMammalianAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA_Cloning Vector
Plasmid#41824PurposeAn empty gRNA expression vector, used to create a gRNA to a specific sequence.DepositorHas serviceCloning Grade DNATypeEmpty backboneUseCRISPRTagsAflII site for Gibson AssemblyExpressionMammalianAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV8)
Viral prep#44362-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cas9
Plasmid#50661PurposeDoxycycline-inducible lentiviral expression of SpCas9DepositorInserthumanized S. pyogenes Cas9
UseCRISPR and LentiviralTags3xFLAGExpressionMammalianPromoterTet ONAvailable sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCW-Cas9
Plasmid#50661PurposeDoxycycline-inducible lentiviral expression of SpCas9DepositorInserthumanized S. pyogenes Cas9
UseCRISPR and LentiviralTags3xFLAGExpressionMammalianPromoterTet ONAvailable sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by