168,579 results
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Plasmid#119236PurposePlasmid encodes full-length human Tie1 gene fused in-frame with Cherry cloned in lentiviral vector.DepositorAvailable SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only
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pSpCas9(BB)-2A-GFP (PX458)
Plasmid#48138PurposeCas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorHas ServiceCloning Grade DNAInserthSpCas9
UseCRISPRTags3XFLAG and GFPExpressionMammalianPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2
Plasmid#52961PurposeReplaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS-NSAvailable SinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-mt-mKeima
Plasmid#131626Purposelentiviral expression of mitochondrial mKeima for generation of stable cell linesDepositorInsertmt-mKeima
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Laconic/pcDNA3.1(-)
Plasmid#44238Purposea genetically-encoded sensor for lactate concentration, transport and metabolic fluxDepositorInsertLaconic (LACtate Optical Nano Indicator from CECs)
UseLactate biosensorExpressionMammalianPromoterCMVAvailable SinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003-sfGFP
Plasmid#133449PurposeExpresses SpyCatcher003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyCatcher003-sfGFP
TagsHis6 and TEV siteExpressionBacterialPromoterT5Available SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSV-Rev
Plasmid#12253Purpose3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)DepositorAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMDLg/pRRE
Plasmid#12251Purpose3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)DepositorInsertHIV-1 GAG/POL
UseLentiviral; PackagingExpressionMammalianAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9-Blast
Plasmid#52962PurposeExpresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.DepositorHas ServiceLentiviral PrepInsertsCas9
Blasticidin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS-NSAvailable SinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-Chimeric_BB-CBh-hSpCas9
Plasmid#42230PurposeA human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.DepositorArticleHas ServiceCloning Grade DNAInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSpliceExpress
Plasmid#32485DepositorTypeEmpty backboneAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIW601-KmCRISPR
Plasmid#98907PurposeK. marxianus CRISPR Plasmid for sgRNA cloningDepositorInsertsCodon optimized Cas9
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsSV40ExpressionYeastAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.TBG.PI.eGFP.WPRE.bGH (AAV8)
Viral Prep#105535-AAV8PurposeReady-to-use AAV8 particles produced from pAAV.TBG.PI.eGFP.WPRE.bGH (#105535). In addition to the viral particles, you will also receive purified pAAV.TBG.PI.eGFP.WPRE.bGH plasmid DNA. TBG-driven eGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTBGTagsEGFPAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-pegRNA-GG-acceptor
Plasmid#132777PurposePrime editing in mammalian cellsDepositorInsertExchangeable cassette
ExpressionMammalianMutationSee manuscriptPromoterU6Available SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV8)
Viral Prep#44361-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available SinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV(CAT)T7-SB100
Plasmid#34879DepositorInsertSB100X transposase
ExpressionMammalianPromoterCMVAvailable SinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro
Plasmid#52963PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.DepositorHas ServiceCloning Grade DNAInsertsS. pyogenes sgRNA cassette
Puromycin resistance
UseCRISPR and LentiviralExpressionMammalianPromoterEf1-a and hU6Available SinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6-tevopreq1-GG-acceptor
Plasmid#174038PurposePrime editing in mammalian cellsDepositorTypeEmpty backboneExpressionMammalianPromoterhU6Available SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP (AAV9)
Viral Prep#50465-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-VSV-G
Plasmid#8454PurposeEnvelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).DepositorTypeEmpty backboneExpressionMammalianAvailable SinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only