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Viral Prep#105551-AAV9PurposeReady-to-use AAV9 particles produced from pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (#105551). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 plasmid DNA. Expression of GFP-Cre from CamKII promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsGFPAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only
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pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV5)
Viral Prep#20297-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-dependent)Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-Ngn2-puro
Plasmid#52047Purpose3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoterDepositorInsertNgn2 (Neurog2 Mouse)
UseLentiviralTagsT2A-and puromycin resistant cassetteExpressionMammalianMutationPromotertet-onAvailable sinceApril 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-VSV-G
Plasmid#8454PurposeEnvelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB-MeCP2
Plasmid#110821PurposeImproved dCas9 repressor-dCas9-KRAB-MeCP2DepositorInsertdCas9-KRAB-MeCP2
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV9)
Viral Prep#44361-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.TBG.PI.eGFP.WPRE.bGH (AAV8)
Viral Prep#105535-AAV8PurposeReady-to-use AAV8 particles produced from pAAV.TBG.PI.eGFP.WPRE.bGH (#105535). In addition to the viral particles, you will also receive purified pAAV.TBG.PI.eGFP.WPRE.bGH plasmid DNA. TBF-driven eGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTBGTagsEGFPAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF2465
Plasmid#142946PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertIRF6 (IRF6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV1)
Viral Prep#100854-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-pegRNA-GG-acceptor
Plasmid#132777PurposePrime editing in mammalian cellsDepositorInsertExchangeable cassette
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-mCherry (AAV1)
Viral Prep#114469-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV(CAT)T7-SB100
Plasmid#34879DepositorInsertSB100X transposase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV9)
Viral Prep#44362-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-10xHis-Super TEV
Plasmid#193833PurposeExpresses high-efficiency TEV protease in bacterial cellsInsertTEV protease
UseTags6xHisExpressionBacterialMutationT17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…PromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/8
Plasmid#112864PurposeAAV packaging plasmid expressing Rep/Cap genesDepositorInsertRep2/Cap8
UseAAVTagsExpressionMutationPromoterTruncated P5Available sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
eeBxb1
Plasmid#222339PurposePlasmid for expressing evolved and engineered Bxb1 in the mammalian cellsDepositorInsertCodon-optimized evolved and engineered Bxb1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
murine TCR OTI-2A.pMIG II
Plasmid#52111Purposeretroviral expression of both TCRalpha and TCRbeta using a single vectorDepositorUseRetroviralTagsT2A (from Thosea asigna virus)ExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HRE-dUnaG
Plasmid#124372PurposeUnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signalingDepositorInsertHRE-dUnaG
UseLentiviralTagsPEST-degron and Myc tagExpressionMutationPromoterHRE (HIF responsive element 5x + CMV minimal prom…Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-gDA3m (AAV1)
Viral Prep#208698-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn-GRAB-gDA3m (#208698). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB-gDA3m plasmid DNA. Syn-driven expression of the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRISPRoff-v2.1
Plasmid#167981PurposeExpresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoterDepositorInsertCRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
UseTags2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only